Fourier transform ion cyclotron resonance mass spectrometry (FTMS) developments and applications to the analysis of glycans, peptidoglycans, and proteoglycans are an important component of this research resource. There are two specific aims for this core project: 1. To design, build, test, and apply a vibrationally cooled matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometer with a mass filtering quadrupole, and collision/accumulation linear ion trap in the front-end ion optics. 2. To build a small 4.7 T FTMS system for testing ICR cells, ion optics, and electronics. During the previous grant period, a high-pressure MALDI FT mass spectrometer was constructed and demonstrated to have significant advantages in cooling labile biomolecule ions that are desorbed in the MALDI experiment. The approach has been used extensively in collaborations, providing a good example of the desired """"""""push-pull"""""""" between technological development and collaboration. Although VC-MALDI-FTMS does cool the vibrational excitation and stabilize, it also cools the MALDI adducts formed from matrix and analyte molecules, causing the MS signal to be distributed over additional components, cluttering the spectra and increasing the space charge. In addition, although MALDI ions can be fragmented in this instrument by IRMPD and SORI-CAD, these ergodic methods select the lowest energy pathway, limiting the information content of the fragmentation and preventing cross-ring cleavages of oligosaccharides. Instrument development such as that proposed in this specific aim is hindered because testing new devices and electronics is slow owing to the extensive pump down that is needed in vacuum recycling. To this end, the construction of a """"""""test-bed"""""""" instrument is proposed.

National Institute of Health (NIH)
National Center for Research Resources (NCRR)
Biotechnology Resource Grants (P41)
Project #
Application #
Study Section
Special Emphasis Panel (ZRG1-BCMB-H (40))
Program Officer
Sheeley, Douglas
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Boston University
Schools of Medicine
United States
Zip Code
Hu, Han; Khatri, Kshitij; Zaia, Joseph (2017) Algorithms and design strategies towards automated glycoproteomics analysis. Mass Spectrom Rev 36:475-498
Sethi, Manveen K; Zaia, Joseph (2017) Extracellular matrix proteomics in schizophrenia and Alzheimer's disease. Anal Bioanal Chem 409:379-394
Hu, Han; Khatri, Kshitij; Klein, Joshua et al. (2016) A review of methods for interpretation of glycopeptide tandem mass spectral data. Glycoconj J 33:285-96
Ji, Yuhuan; Bachschmid, Markus M; Costello, Catherine E et al. (2016) S- to N-Palmitoyl Transfer During Proteomic Sample Preparation. J Am Soc Mass Spectrom 27:677-85
Pu, Yi; Ridgeway, Mark E; Glaskin, Rebecca S et al. (2016) Separation and Identification of Isomeric Glycans by Selected Accumulation-Trapped Ion Mobility Spectrometry-Electron Activated Dissociation Tandem Mass Spectrometry. Anal Chem 88:3440-3
Wang, Yun Hwa Walter; Meyer, Rosana D; Bondzie, Philip A et al. (2016) IGPR-1 Is Required for Endothelial Cell-Cell Adhesion and Barrier Function. J Mol Biol 428:5019-5033
Srinivasan, Srimathi; Chitalia, Vipul; Meyer, Rosana D et al. (2015) Hypoxia-induced expression of phosducin-like 3 regulates expression of VEGFR-2 and promotes angiogenesis. Angiogenesis 18:449-62
Yu, Xiang; Sargaeva, Nadezda P; Thompson, Christopher J et al. (2015) In-Source Decay Characterization of Isoaspartate and ?-Peptides. Int J Mass Spectrom 390:101-109
Steinhorn, Benjamin S; Loscalzo, Joseph; Michel, Thomas (2015) Nitroglycerin and Nitric Oxide--A Rondo of Themes in Cardiovascular Therapeutics. N Engl J Med 373:277-80
Walsh, Erica M; Niu, MengMeng; Bergholz, Johann et al. (2015) Nutlin-3 down-regulates retinoblastoma protein expression and inhibits muscle cell differentiation. Biochem Biophys Res Commun 461:293-9

Showing the most recent 10 out of 252 publications