PROTEIN YEAST ISOLATION AT THE MICROSCALE LEVEL, INCORPORATING 2-D GEL ELECTROPHORESIS, WILL BE A CENTRAL COMPONENT IN THIS RESOURCE CENTER FOR THE STRUCTURE DETERMINATION OF PROTEINS IN YEAST CELLS THAT ARE INVOLVED IN A VARIETY OF BIOLOGICAL PROCESSES, INCLUDING DNA REPAIR, THE CELL CYCLE AND SIGNAL TRANSDUCTION. RATHER THAN A GLOBAL DATA BASE STRATEGY OF EXAMINING 1000 OR MORE PROTEINS FROM A CELL LYSATE, THE APPROACH TO BE TAKEN HERE WILL BE A FOCUS ON PARTICULAR BIOLOGICAL QUESTIONS. THUS, APPROPRIATE ENRICHMENT VIA AFFINITY SELECTION PROCEDURES WILL BE UTILIZED PRIOR TO 2-D GEL OPERATION. AFTER SEPARATION, THE SPOTS WILL BE BLOTTED ON MEMBRANES AND THEN DIGESTED FOR LC/CE/ESI/MS/MS ANALYSIS.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
1P41RR011823-01
Application #
5226017
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1996
Total Cost
Indirect Cost
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