This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.We are conducting experiments using stable isotope labeling and mass spectrometry to measure protein turnover in C. elegans. The turnover of protein is measured by comparing the incorporation of 15N into proteins over different time intervals. These experiments are being conducted with the adult glp-4 strain in the background of all strains to suppress worm progeny production and therefore keep all animals at the same life stage. This methodology can be used to look at the turnover of proteins in multiple organisms during development and other processes. We specifically are interested in measuring turnover of proteins in the insulin/IGF-1 signaling pathway in C. elegans while overexpressing the ligands of the pathway. Proteins in the insulin/IGF-1 signaling pathway have been shown to be involved in longevity, metabolism and development. By looking at the turnover of proteins in this pathway during ligand overexpression, we hope to improve our understanding of the mechanisms relating to insulin signaling.
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