This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Accurate chromosome segregation is required for propagation of all cells. Errors in this process are implicated in oncogenesis, developmental errors, and cell death. Crucial to proper partitioning of the chromosomes during cell division is the accurate duplication, assembly and regulation of the centrosome, which is responsible for forming the complex protein structure that segregates the genetic material. The spindle pole body (SPB) in yeast is the best characterized centrosome. Understanding its structure and assembly will provide important insights into the essential process of distributing the chromosomes in each cell division. The Sali lab developed the IMP software package to construct detailed models of the architecture of large macromolecular protein complexes. We propose to use IMP in an iterative process to construct a high resolution model of the central core of the yeast SPB. The distance constraints for the construction of the model will rely on currently available data and data generated by FRET and cross-linking analysis.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
2P41RR011823-16
Application #
8365787
Study Section
Special Emphasis Panel (ZRG1-CB-L (40))
Project Start
2011-09-01
Project End
2012-06-30
Budget Start
2011-09-01
Budget End
2012-06-30
Support Year
16
Fiscal Year
2011
Total Cost
$28,839
Indirect Cost
Name
University of Washington
Department
Biochemistry
Type
Schools of Medicine
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195
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