This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Background: For established cellulose-based H2-producing co-culture systems, modest H2 production has been achieved in bioreactors with syntrophic microorganisms. However, the metabolic interactions between the organisms are poorly understood. This shortcoming hinders system optimization for improving H2 production efficiency. Methods: We achieved a co-culture system for H2 production based on cellulosic materials with Clostridium cellulolyticum H10 and Rhodopseudomonas palustris CGA584. Dark fermentation by H10 decomposes cellulose into small organic acids, CO2 and H2. CGA584 cannot use cellulose or glucose directly, though it can further degrade fermentation products produced and released in large quantities by H10, yielding additional H2. A stable co-culture was established and the kinetics and stoichiometry of the syntrophic growth were monitored. We have identified several of the organic acids produced and released by H10, but it is important to determine whether other, previously unidentified metabolites are released by H10 and thus become available for uptake and metabolism by CGA584. Therefore, we propose to use AMS to determine the presence or absence of additional metabolites released by H10 that are not detectable using HPLC with UV-Vis and refractive index detection. Results from these experiments will provide the basis for further characterization of the metabolic interaction between H10 and CGA584 in the production of H2 as a biofuel using cellulose as a feedstock. Expected Results and Future Directions: We anticipate that we be able to determine whether previously unidentified metabolites are secreted by H10, which will allow us to develop methods to identify those metabolites and monitor them in future experiments. These experiments will also confirm that metabolites secreted by H10 are the sole carbon source for CGA584. This information, and data from the additional experiments it will enable, will provide a basis for computational modeling of the interaction between H10 and CGA584 in the production of H2 fuel as a result of cellulose degradation. A valid computational model can then be used to predict optimal growth conditions for biofuel production.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR013461-13
Application #
8362770
Study Section
Special Emphasis Panel (ZRG1-BCMB-K (40))
Project Start
2011-06-01
Project End
2012-05-31
Budget Start
2011-06-01
Budget End
2012-05-31
Support Year
13
Fiscal Year
2011
Total Cost
$234,050
Indirect Cost
Name
Lawrence Livermore National Laboratory
Department
Biology
Type
Organized Research Units
DUNS #
827171463
City
Livermore
State
CA
Country
United States
Zip Code
94550
Wan, Debin; Yang, Jun; Barnych, Bogdan et al. (2017) A new sensitive LC/MS/MS analysis of vitamin D metabolites using a click derivatization reagent, 2-nitrosopyridine. J Lipid Res 58:798-808
Stornetta, Alessia; Zimmermann, Maike; Cimino, George D et al. (2017) DNA Adducts from Anticancer Drugs as Candidate Predictive Markers for Precision Medicine. Chem Res Toxicol 30:388-409
Wang, Si-Si; Zimmermann, Maike; Zhang, Hongyong et al. (2017) A diagnostic microdosing approach to investigate platinum sensitivity in non-small cell lung cancer. Int J Cancer 141:604-613
Wang, Zhican; Fang, Ying; Teague, Juli et al. (2017) In Vitro Metabolism of Oprozomib, an Oral Proteasome Inhibitor: Role of Epoxide Hydrolases and Cytochrome P450s. Drug Metab Dispos 45:712-720
Kim, Jeffrey; Stewart, Benjamin; Weiss, Robert H (2016) Extraction and Quantification of Tryptophan and Kynurenine from Cultured Cells and Media Using a High Performance Liquid Chromatography (HPLC) System Equipped with an Ultra-Sensitive Diode Array Detector. Bio Protoc 6:
Pan, Amy; Zhang, Hongyong; Li, Yuanpei et al. (2016) Disulfide-crosslinked nanomicelles confer cancer-specific drug delivery and improve efficacy of paclitaxel in bladder cancer. Nanotechnology 27:425103
Wang, Sisi; Zhang, Hongyong; Scharadin, Tiffany M et al. (2016) Molecular Dissection of Induced Platinum Resistance through Functional and Gene Expression Analysis in a Cell Culture Model of Bladder Cancer. PLoS One 11:e0146256
McCartt, A D; Ognibene, T; Bench, G et al. (2015) Measurements of Carbon-14 With Cavity Ring-Down Spectroscopy. Nucl Instrum Methods Phys Res B 361:277-280
Cai, Hong; Scott, Edwina; Kholghi, Abeer et al. (2015) Cancer chemoprevention: Evidence of a nonlinear dose response for the protective effects of resveratrol in humans and mice. Sci Transl Med 7:298ra117
Tomlinson, Ben; Lin, Tzu-yin; Dall'Era, Marc et al. (2015) Nanotechnology in bladder cancer: current state of development and clinical practice. Nanomedicine (Lond) 10:1189-201

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