This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Construction of the complete Modulated Gradient Coil Driver (MGCD) for c.w.-ESR microimaging continues, as described in our most recent (2003) progress report. Fabrication, test and implementation of a prototype MGCD output section electronic assembly has been completed, and this subsystem has been integrated into our 3-D c.w. ESR imaging microscope/spectrometer which has achieved resolution to date of 10?m x 10?m x 30?m (described in a recently-accepted Rev. Sci. Inst. article). The primary incentive for continuing development of the MGCD has been the readily evident need for real-time waveform generation hardware which is capable of providing rapidly-updated, complex modulation of imaging resonator gradient magnetic fields. Our MGCD hardware-based waveform generator, in its final version, will take the place of the existing software-generated waveform subsystem. By incorporating pre-defined coil excitation functions and a capability of preloading and rapidly updating individual coil drive levels, the deadtime between discrete voxel acquisitions will be reduced by an order of magnitude or more relative to our present interpretive computer-driven excitation system. Computer overhead will also be reduced by an order of magnitude or better, since the CPU will no longer be required to calculate and update the gradient coil currents in real-time. An important secondary feature of the MGCD system module is that it includes common-mode offsetting of B0 as necessary to augment spectrometer AFC action. Anticipating possible future investigation of stochastic CW imaging techniques, we have also designed into the MGCD sufficient processing and driver bandwidth to permit gradient field modulation by fast pseudo-random sequences. Presently, in Phase II, we have completed construction of the main signal processing circuit board, which will be debugged and soon installed in the c.w.-ESR microscope with a companion wire-wrapped version of the function generator/controller electronics subsystems as we proceed with the 3-D c.w. microimaging project.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
2P41RR016292-11
Application #
8363951
Study Section
Special Emphasis Panel (ZRG1-BCMB-K (40))
Project Start
2011-09-01
Project End
2012-08-31
Budget Start
2011-09-01
Budget End
2012-08-31
Support Year
11
Fiscal Year
2011
Total Cost
$482
Indirect Cost
Name
Cornell University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
872612445
City
Ithaca
State
NY
Country
United States
Zip Code
14850
Jain, Rinku; Vanamee, Eva S; Dzikovski, Boris G et al. (2014) An iron-sulfur cluster in the polymerase domain of yeast DNA polymerase ?. J Mol Biol 426:301-8
Pratt, Ashley J; Shin, David S; Merz, Gregory E et al. (2014) Aggregation propensities of superoxide dismutase G93 hotspot mutants mirror ALS clinical phenotypes. Proc Natl Acad Sci U S A 111:E4568-76
Georgieva, Elka R; Borbat, Peter P; Ginter, Christopher et al. (2013) Conformational ensemble of the sodium-coupled aspartate transporter. Nat Struct Mol Biol 20:215-21
Airola, Michael V; Sukomon, Nattakan; Samanta, Dipanjan et al. (2013) HAMP domain conformers that propagate opposite signals in bacterial chemoreceptors. PLoS Biol 11:e1001479
Airola, Michael V; Huh, Doowon; Sukomon, Nattakan et al. (2013) Architecture of the soluble receptor Aer2 indicates an in-line mechanism for PAS and HAMP domain signaling. J Mol Biol 425:886-901
Sun, Yan; Zhang, Ziwei; Grigoryants, Vladimir M et al. (2012) The internal dynamics of mini c TAR DNA probed by electron paramagnetic resonance of nitroxide spin-labels at the lower stem, the loop, and the bulge. Biochemistry 51:8530-41
Yu, Renyuan Pony; Darmon, Jonathan M; Hoyt, Jordan M et al. (2012) High-Activity Iron Catalysts for the Hydrogenation of Hindered, Unfunctionalized Alkenes. ACS Catal 2:1760-1764
Dzikovski, Boris; Tipikin, Dmitriy; Freed, Jack (2012) Conformational distributions and hydrogen bonding in gel and frozen lipid bilayers: a high frequency spin-label ESR study. J Phys Chem B 116:6694-706
Gaffney, Betty J; Bradshaw, Miles D; Frausto, Stephen D et al. (2012) Locating a lipid at the portal to the lipoxygenase active site. Biophys J 103:2134-44
Maeda, Kiminori; Lodge, Matthew T J; Harmer, Jeffrey et al. (2012) Electron tunneling in lithium-ammonia solutions probed by frequency-dependent electron spin relaxation studies. J Am Chem Soc 134:9209-18

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