This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Escherichia coli cells containing artificial subcellular structures (bundles of the actin-like filament protein ParM, tobacco mosaic virus particles and polyester inclusions) will be examined using X-ray tomography to investigate bacterial subcellular organisation and the potential use of such structures as cloneable labels.
We aim to use X-ray tomography to investigate the subcellular organisation of Escherichia coli bacteria containing three different artificial subcellular structures: bundles of ParM filaments produced by overexpression of the actin-like ParM protein 2;tobacco mosaic virus (TMV) particles;and polyester inclusions generated by a poly-hydroxyalkanoate synthase enzyme. The latter two structures could potentially be adapted as cloneable labels if they prove to be identifiable in X-ray tomographic images.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR019664-07
Application #
8362749
Study Section
Special Emphasis Panel (ZRG1-BST-K (40))
Project Start
2011-05-01
Project End
2012-04-30
Budget Start
2011-05-01
Budget End
2012-04-30
Support Year
7
Fiscal Year
2011
Total Cost
$19,596
Indirect Cost
Name
University of California San Francisco
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
094878337
City
San Francisco
State
CA
Country
United States
Zip Code
94143
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Le Gros, Mark A; McDermott, Gerry; Cinquin, Bertrand P et al. (2014) Biological soft X-ray tomography on beamline 2.1 at the Advanced Light Source. J Synchrotron Radiat 21:1370-7
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Isaacson, Samuel A; Larabell, Carolyn A; Le Gros, Mark A et al. (2013) The influence of spatial variation in chromatin density determined by X-ray tomograms on the time to find DNA binding sites. Bull Math Biol 75:2093-117
McDermott, Gerry; Le Gros, Mark A; Larabell, Carolyn A (2012) Visualizing cell architecture and molecular location using soft x-ray tomography and correlated cryo-light microscopy. Annu Rev Phys Chem 63:225-39

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