The long term objective of the Functional Genomics and Bioinformatics Core Laboratory (FGBCL) is to provide a variety of genomics-based assays, basic proteomic measurements, and bioinformatic analyses in a cost-effective and efficient manner, therefore maximizing the availability of these important tools for all five individual UW SBRP investigators. The FGBCL will furnish microarray-based gene-centric transcript expression analysis (using Affymetrix arrays and/or NimbleGen arrays), ELISA-based measurements, quantitative gene expression evaluations, and bioinformatic analyses of data including the topGO weight method, gene set analysis, and PAINT. The FGBCL services to four investigators (Drs Furlong, Checkoway, Zhang, and Gallagher) will support the specific Biomedical Research objective through """"""""the identification and development of methods and tools to improve our understanding of the relationship of exposure to hazardous substances and health"""""""". Furthermore, the FGBCL services offered to Drs Stand/Doty (Project #5) and Gallagher are directed toward the Remediation Research objectives """"""""initiating research on new and innovative remediation strategies and technologies that reduce bioavailability and/or toxicity of hazardous substances"""""""" and """"""""development of new, non-invasive methods to characterize hazardous waste sites and for the long-term assessment of the effectiveness of remedial actions"""""""", respectively. The FGBCL services also supports other goals and objectives of this RFA by """"""""encouraging the use of the technological advances, as appropriate, to support multi-project, interdisciplinary research programs"""""""". Specifically, the FGBCL will offer state-of-the-art technologies including microarray-based analyses for genomic-related studies as well as bioinformatic analyses of microarray- and proteomic-derived data. The FGBCL support work will contribute directly to the central theme of this program which is to """"""""focus on a wide range of molecular biology- and proteomic-based measurements that may be predictive of: exposure to toxicants;impaired physiologic function;and/or unusual susceptibility to damage from toxic agents that occur in the environment, particularly those that are commonly present at hazardous waste sites.""""""""

Public Health Relevance

The Functional Genomics and Bioinformatics Core Laboratory services provided to these Superfund projects will develop methods and technologies to detect and monitor hazardous substances;will create and validate techniques for the detection and evaluation of the effect on human health of hazardous substances;and will investigate basic biological methods to reduce the amount of hazardous substances in our environment.

National Institute of Health (NIH)
National Institute of Environmental Health Sciences (NIEHS)
Hazardous Substances Basic Research Grants Program (NIEHS) (P42)
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Meador, James P; Yeh, Andrew; Gallagher, Evan P (2017) Determining potential adverse effects in marine fish exposed to pharmaceuticals and personal care products with the fish plasma model and whole-body tissue concentrations. Environ Pollut 230:1018-1029
Chang, Yu-Chi; Cole, Toby B; Costa, Lucio G (2017) Behavioral Phenotyping for Autism Spectrum Disorders in Mice. Curr Protoc Toxicol 72:11.22.1-11.22.21
Roqué, Pamela J; Costa, Lucio G (2017) Co-Culture of Neurons and Microglia. Curr Protoc Toxicol 74:11.24.1-11.24.17
Wang, Hao; Engstrom, Anna K; Xia, Zhengui (2017) Cadmium impairs the survival and proliferation of cultured adult subventricular neural stem cells through activation of the JNK and p38 MAP kinases. Toxicology 380:30-37
Yeh, Andrew; Marcinek, David J; Meador, James P et al. (2017) Effect of contaminants of emerging concern on liver mitochondrial function in Chinook salmon. Aquat Toxicol 190:21-31
Mills, Margaret G; Gallagher, Evan P (2017) A targeted gene expression platform allows for rapid analysis of chemical-induced antioxidant mRNA expression in zebrafish larvae. PLoS One 12:e0171025
Costa, Lucio G; Cole, Toby B; Coburn, Jacki et al. (2017) Neurotoxicity of traffic-related air pollution. Neurotoxicology 59:133-139
Costa, Lucio G; Cole, Toby B; Garrick, Jacqueline M et al. (2017) Metals and Paraoxonases. Adv Neurobiol 18:85-111
Al-Lozi, Amal; Nielsen, Susan Searles; Hershey, Tamara et al. (2017) Cognitive control dysfunction in workers exposed to manganese-containing welding fume. Am J Ind Med 60:181-188
Criswell, Susan R; Nielsen, Susan Searles; Warden, Mark et al. (2017) [18F]FDOPA positron emission tomography in manganese-exposed workers. Neurotoxicology :

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