The Analytical Core is a central resource composed of two laboratories that facilitate-development and application of modern analytical methods to solve key problems encountered by the components of the Superfund Program. The first is the Superfund Analytical Laboratory which provides a range of instrument services with emphasis on mass spectrometry and chromatography and small molecule analysis. The second is the Accelerator Mass Spectrometry Facility with 2 AMS instruments at Lawrence Livermore National Laboratory for ultra trace analysis. Proteomic analysis is largely provided in Core B (formerly Core C) although the instrumentation in Core A is available for proteomic work. The Analytical Core provides walk-up instrumentation as well as services and research collaborations to the projects. The services include assistance with other campus facilities such as nuclear magnetic resonance (NMR). Core A advances many analytical technologies, including preparation, throughput and accuracy by developing robotic procedures, sample clean up, approaches to sample preparation and derivatization. Core A supports the development of both global and pathway selective metabolomic techniques and their applications to hypothesis generation and testing as well as for biomarker development. It carries out bioassay driven fractionation of hazardous mixtures, in collaboration with the projects and provides education for Superfund scientists in analytical chemistry. The LLNL component provides access to a variety of advanced instruments but focuses on the sub attomole (i.e. 10[-18]) sensitivity of AMS in support of projects using [14]C as a heavy isotopes. This technique for the first time allows human monitoring of metabolism of hazardous chemicals at environmentally realistic levels using exceptionally low levels of tracers. Priority targets for AMS are to quantify human metabolism of the insecticide permethrin and the personal care product triclocarban. Core A scientists provide one-on-one assistance in analytical chemistry, data generation and analysis and on going formal training in analytical techniques for Superfund researchers. Thus, Core A provides the broad analytical support necessary for developing and using biomarkers of exposure and effect.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Hazardous Substances Basic Research Grants Program (NIEHS) (P42)
Project #
5P42ES004699-27
Application #
8450327
Study Section
Special Emphasis Panel (ZES1-LWJ-M)
Project Start
Project End
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
27
Fiscal Year
2013
Total Cost
$273,519
Indirect Cost
$96,813
Name
University of California Davis
Department
Type
DUNS #
047120084
City
Davis
State
CA
Country
United States
Zip Code
95618
Patchin, Esther Shin; Anderson, Donald S; Silva, Rona M et al. (2016) Size-Dependent Deposition, Translocation, and Microglial Activation of Inhaled Silver Nanoparticles in the Rodent Nose and Brain. Environ Health Perspect 124:1870-1875
Gee, Shirley J; Kennedy, Ivan R; Lee, N Alice et al. (2016) Immunoanalysis for environmental monitoring and human health. Anal Bioanal Chem 408:5959-61
Bahl, Christopher D; Hvorecny, Kelli L; Morisseau, Christophe et al. (2016) Visualizing the Mechanism of Epoxide Hydrolysis by the Bacterial Virulence Enzyme Cif. Biochemistry 55:788-97
Chapman, Christopher A R; Chen, Hao; Stamou, Marianna et al. (2016) Mechanisms of Reduced Astrocyte Surface Coverage in Cortical Neuron-Glia Co-cultures on Nanoporous Gold Surfaces. Cell Mol Bioeng 9:433-442
Das, Gautom K; Anderson, Donald S; Wallis, Chris D et al. (2016) Novel multi-functional europium-doped gadolinium oxide nanoparticle aerosols facilitate the study of deposition in the developing rat lung. Nanoscale 8:11518-30
Ahn, Ki Chang; Ranganathan, Anupama; Bever, Candace S et al. (2016) Detection of the Antimicrobial Triclosan in Environmental Samples by Immunoassay. Environ Sci Technol 50:3754-61
Ronjat, Michel; Feng, Wei; Dardevet, Lucie et al. (2016) In cellulo phosphorylation induces pharmacological reprogramming of maurocalcin, a cell-penetrating venom peptide. Proc Natl Acad Sci U S A 113:E2460-8
Wu, Xianai; Yang, Jun; Morisseau, Christophe et al. (2016) 3,3',4,4',5-Pentachlorobiphenyl (PCB 126) Decreases Hepatic and Systemic Ratios of Epoxide to Diol Metabolites of Unsaturated Fatty Acids in Male Rats. Toxicol Sci 152:309-22
Croes, Kim; Debaillie, Pieterjan; Van den Bril, Bo et al. (2016) Assessment of estrogenic activity in PM₁₀ air samples with the ERE-CALUX bioassay: Method optimization and implementation at an urban location in Flanders (Belgium). Chemosphere 144:392-8
Ren, Qian; Ma, Min; Ishima, Tamaki et al. (2016) Gene deficiency and pharmacological inhibition of soluble epoxide hydrolase confers resilience to repeated social defeat stress. Proc Natl Acad Sci U S A 113:E1944-52

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