Project 4: Meta-omics of Microbial Communities Involved in Bioremediation. Trichloroethene (TCE), a common chlorinated solvent, and 1,4-dioxane (dioxane), a solvent stabilizer, both are frequent groundwater contaminants at Superfund sites. Although microbial degradation reactions can effectively transform these contaminants, lack of a holistic and ecologically relevant understanding of the interspecies complexity of subsurface microbial communities impedes the application of robust and effective in situ bioremediation of these compounds. The overall goal of this research is to identify and examine the community-level metabolic interactions that shape the biodegradation capabilities of bioremediating communities. The fundamental understanding of microbial communities from a systems microbiology point of view will be developed by utilizing community-scale tools on two dissimilar model communities that function within different redox environments, i.e. anaerobic and aerobic, to remediate TCE and dioxane, respectively. We hypothesize that important interspecies interactions and keystone functions that regulate multiple community behaviors will be identified by applying an integrated meta-omics based approach, which in turn will provide insights to facilitate engineering efficient bioremediation processes. DNA-based stable isotope probing will be used to distinguish and identify functional organisms and the important interdependent relationships within communities capable of biodegrading TCE or dioxane. Targeted metagenomic analyses will then be employed to reveal the metabolic and functional diversity of interdependent biodegrading organisms and their supporting or inhibiting partners in the communities. Comprehensive metatranscriptomic analyses based on mRNA-targeted microarrays will be employed to investigate the global gene expression in the communities under different environmental perturbations in order to reveal microbial behaviors in response to the changes. In order to design diagnostic tools that can be applied to query environmental samples at TCE and dioxane-contaminated sites, we will identify and validate quantitative biomarkers for key metabolic as well as interacting genes using quantitative expression analyses in conjunction with community metabolite-analyses. Finally, we will apply the diagnostic tools developed throughout the research to examine environmental samples to assess microbial behaviors and interactions in these samples in order to guide and optimize in situ bioremediation strategies.
TCE and dioxane, suspected human carcinogens, are common groundwater contaminants at Superfund sites. This research focuses on gaining a holistic understanding and developing novel biomarkers of the community-level metabolism and interactions that shape the bioremediation capabilities of subsurface microbial communities. Results from this research will meet SRP research goals by facilitating the assessment, design and optimization of in situ bioremediation of TCE and dioxane-contaminated sites.
|Whitehead, Todd P; Adhatamsoontra, Praphopphat; Wang, Yang et al. (2017) Home remodeling and risk of childhood leukemia. Ann Epidemiol 27:140-144.e4|
|Nardone, Anthony; Ferreccio, Catterina; Acevedo, Johanna et al. (2017) The impact of BMI on non-malignant respiratory symptoms and lung function in arsenic exposed adults of Northern Chile. Environ Res 158:710-719|
|Burgos-Barragan, Guillermo; Wit, Niek; Meiser, Johannes et al. (2017) Mammals divert endogenous genotoxic formaldehyde into one-carbon metabolism. Nature 548:549-554|
|de la Rosa, Rosemarie; Steinmaus, Craig; Akers, Nicholas K et al. (2017) Associations between arsenic (+3 oxidation state) methyltransferase (AS3MT) and N-6 adenine-specific DNA methyltransferase 1 (N6AMT1) polymorphisms, arsenic metabolism, and cancer risk in a chilean population. Environ Mol Mutagen 58:411-422|
|Men, Yujie; Yu, Ke; Bælum, Jacob et al. (2017) Metagenomic and Metatranscriptomic Analyses Reveal the Structure and Dynamics of a Dechlorinating Community Containing Dehalococcoides mccartyi and Corrinoid-Providing Microorganisms under Cobalamin-Limited Conditions. Appl Environ Microbiol 83:|
|Mao, Xinwei; Polasko, Alexandra; Alvarez-Cohen, Lisa (2017) Effects of Sulfate Reduction on Trichloroethene Dechlorination by Dehalococcoides-Containing Microbial Communities. Appl Environ Microbiol 83:|
|Bessonneau, Vincent; Pawliszyn, Janusz; Rappaport, Stephen M (2017) The Saliva Exposome for Monitoring of Individuals' Health Trajectories. Environ Health Perspect 125:077014|
|Edmands, William M B; Petrick, Lauren; Barupal, Dinesh K et al. (2017) compMS2Miner: An Automatable Metabolite Identification, Visualization, and Data-Sharing R Package for High-Resolution LC-MS Data Sets. Anal Chem 89:3919-3928|
|Gunier, Robert B; Kang, Alice; Hammond, S Katharine et al. (2017) A task-based assessment of parental occupational exposure to pesticides and childhood acute lymphoblastic leukemia. Environ Res 156:57-62|
|Hall, Emily M; Acevedo, Johanna; López, Francisca González et al. (2017) Hypertension among adults exposed to drinking water arsenic in Northern Chile. Environ Res 153:99-105|
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