Core C: Genomics and Analytical Chemistry. Core C comprises the combined expertise of Drs. Christine Skibola and Evan Williams, the two co-leaders ofthe Core, who have broad experience in the application of cutting-edge technologies to support Projects 1-6 ofthe Berkeley Superfund Research Program (SRP). The overall goals of the SRP are to use 'omic'technologies and modern analytical methods to develop biological markers for application in human studies and site remediation to allow better detection and remediation of Superfund priority chemicals and emerging contaminants in the environment;and to better evaluate the risk they pose to human health. Core C is set up specifically for the purposes of providing the laboratory infrastructure and expertise for Projects 1-6 to achieve these goals. To meet many of the Aims of Projects 1- 4 and ensure their success, investigators require access, know-how and technical support to apply cuttingedge technologies such as functional genomics, transcriptomics, proteomics, genetics and epigenetics. Project 3 is an epidemiological study that requires effective handling and management of biological samples so that these technologies can be applied. To accomplish these goals, detailed collection and storage protocols have been designed and the Core will provicie facilities for the genetic and proteomic studies that will be conducted in Projects 1-4. Projects 3 and 5 will also require sophisticated mass spectrometry analyses that will be provided by the QB3/Chemistry Mass Spectrometry Facility. The Core will also provide support for high-throughput analysis of toxic metabolites for Project 6 through the Berkeley Screening Center.
The specific aims of Core C are to: 1) process, maintain and store biological samples and cell lines; 2) provide facilities and methodologies for gene expression and epigenetic studies;3) provide analytical support for proteomic studies, for the identification of proteins and organic and inorganic compounds through our QBS/Chemistry Mass Spectrometry Facility, and toxicity screening support;4) provide facilities and methodologies to analyze genetic polymorphisms;and, 5) provide sequencing capabilities at our QB3 DNA sequencing facility. The Core will provide expertise and analytical support in all required areas that, along with the computational biology skills of Core D, will allow for the successful completion of Projects 1-6 of the Berkeley SRP.

Public Health Relevance

The Genomics and Analytical Chemistry Core will provide a centralized source of specialized facilities and equipment, services, well-tested collection and storage protocols, and expert technical support using the latest -omics technologies and analytical instruments for Berkeley Superfund Research Program project investigators. These services will greatly enhance the success of Projects 1-6 investigators to achieve their overall goals.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Hazardous Substances Basic Research Grants Program (NIEHS) (P42)
Project #
5P42ES004705-26
Application #
8461219
Study Section
Special Emphasis Panel (ZES1-SET-V)
Project Start
Project End
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
26
Fiscal Year
2013
Total Cost
$217,505
Indirect Cost
$67,871
Name
University of California Berkeley
Department
Type
DUNS #
124726725
City
Berkeley
State
CA
Country
United States
Zip Code
94704
Smith, Allan H; Marshall, Guillermo; Roh, Taehyun et al. (2018) Lung, Bladder, and Kidney Cancer Mortality 40?Years After Arsenic Exposure Reduction. J Natl Cancer Inst 110:241-249
Castriota, Felicia; Acevedo, Johanna; Ferreccio, Catterina et al. (2018) Obesity and increased susceptibility to arsenic-related type 2 diabetes in Northern Chile. Environ Res 167:248-254
Rothman, Nathaniel; Zhang, Luoping; Smith, Martyn T et al. (2018) Formaldehyde, Hematotoxicity, and Chromosomal Changes-Response. Cancer Epidemiol Biomarkers Prev 27:120-121
Yik-Sham Chung, Clive; Timblin, Greg A; Saijo, Kaoru et al. (2018) Versatile Histochemical Approach to Detection of Hydrogen Peroxide in Cells and Tissues Based on Puromycin Staining. J Am Chem Soc 140:6109-6121
Rappaport, Stephen M (2018) Redefining environmental exposure for disease etiology. NPJ Syst Biol Appl 4:30
Tachachartvanich, Phum; Sangsuwan, Rapeepat; Ruiz, Heather S et al. (2018) Assessment of the Endocrine-Disrupting Effects of Trichloroethylene and Its Metabolites Using in Vitro and in Silico Approaches. Environ Sci Technol 52:1542-1550
Guyton, Kathryn Z; Rieswijk, Linda; Wang, Amy et al. (2018) Key Characteristics Approach to Carcinogenic Hazard Identification. Chem Res Toxicol :
Roh, Taehyun; Steinmaus, Craig; Marshall, Guillermo et al. (2018) Age at Exposure to Arsenic in Water and Mortality 30-40 Years After Exposure Cessation. Am J Epidemiol 187:2297-2305
Daniels, Sarah I; Chambers, John C; Sanchez, Sylvia S et al. (2018) Elevated Levels of Organochlorine Pesticides in South Asian Immigrants Are Associated With an Increased Risk of Diabetes. J Endocr Soc 2:832-841
Guyton, Kathryn Z; Rusyn, Ivan; Chiu, Weihsueh A et al. (2018) Application of the key characteristics of carcinogens in cancer hazard identification. Carcinogenesis 39:614-622

Showing the most recent 10 out of 629 publications