Project 3: Abstract Arsenic (As) is the number one environmental chemical of concern with regard to human health in the US. Epidemiological studies have shown that exposure to As increases lung disease, including pneumonia, and chronic obstructive pulmonary disease. In studies on experimental animals low levels of As inhibit the ability of the innate immune system to eliminate respiratory infections, and down regulate the expression of innate immune genes, but the molecular mechanisms whereby As inhibits the innate immune system is unknown. Moreover, the effects of inorganic versus organic As on the innate immune system is unknown. Accordingly, the goal of this application is to test the hypothesis that inorganic and organic forms have differential, dose dependent effects on the Pseudomonas aeruginosa (Pa) infections in the lung by adversely affecting the innate immune response. This will be investigated in two specific aims.
Specific Aim #1 will test the hypothesis that arsenite, monomethylarsonic acid (MMA) and dimetheylarsinic acid (DMA) have differential, dose dependent effects on the secretion of proinflammatory cytokines by human bronchial epithelial (HBE) cells and macrophages in response to Pa. Studies will be conducted to examine the effects of arsenite, MMA and DMA, at levels relevant to the US population, on cytokine production by HBE cells and macrophages exposed to Pa.
Specific Aim #2 will test the hypothesis that arsenite, MMA and DMA have differential, dose dependent effects on the expression of proinflammatory cytokine genes by HBE cells and macrophages in response to Pa by selectively regulating microRNA (miRNA) expression. Using advanced bioinformatic and molecular biological approaches, studies will be conducted to elucidate how miRNAs regulated by As modulate the inflammatory response to Pa. These studies will provide novel information regarding the dose and species dependent effects of arsenite, MMA and DMA, at levels relevant to the US population, on the immune response to Ps, a pathogen that causes significant morbidity and mortality in the US, as well as provide novel insight into the molecular mechanism(s) whereby As modulates the innate immune response of the human lung to Pa.

Public Health Relevance

Project 3: Narrative Environmental exposure to arsenic is associated with increased risk of lung disease. The goal of this project is to examine the effects of inorganic versus organic arsenic on the immune response of the lungs to infection by P. aeruginosa, a common cause of respiratory infections. These studies will provide novel information that will be useful to stakeholders, including the FDA, in discussions on food safety standards.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Hazardous Substances Basic Research Grants Program (NIEHS) (P42)
Project #
3P42ES007373-19A1S1
Application #
8881879
Study Section
Special Emphasis Panel (ZES1-LKB-K)
Project Start
2014-07-01
Project End
2015-03-31
Budget Start
2014-07-01
Budget End
2015-03-31
Support Year
19
Fiscal Year
2014
Total Cost
$1,200
Indirect Cost
$89
Name
Dartmouth College
Department
Type
DUNS #
041027822
City
Hanover
State
NH
Country
United States
Zip Code
03755
Chen, Celia Y; Borsuk, Mark E; Bugge, Deenie M et al. (2014) Benthic and pelagic pathways of methylmercury bioaccumulation in estuarine food webs of the northeast United States. PLoS One 9:e89305
Shaw, Joseph R; Hampton, Thomas H; King, Benjamin L et al. (2014) Natural selection canalizes expression variation of environmentally induced plasticity-enabling genes. Mol Biol Evol 31:3002-15
Sverrisson, Einar F; Zens, Michael S; Fei, Dennis Liang et al. (2014) Clinicopathological correlates of Gli1 expression in a population-based cohort of patients with newly diagnosed bladder cancer. Urol Oncol 32:539-45
Taylor, Vivien F; Bugge, Deenie; Jackson, Brian P et al. (2014) Pathways of CH3Hg and Hg ingestion in benthic organisms: an enriched isotope approach. Environ Sci Technol 48:5058-65
Torres, Iviana M; Patankar, Yash R; Shabaneh, Tamer B et al. (2014) Acidosis potentiates the host proinflammatory interleukin-1? response to Pseudomonas aeruginosa infection. Infect Immun 82:4689-97
Gosse, Julie A; Taylor, Vivien F; Jackson, Brian P et al. (2014) Monomethylated trivalent arsenic species disrupt steroid receptor interactions with their DNA response elements at non-cytotoxic cellular concentrations. J Appl Toxicol 34:498-505
Kwon, Sae Yun; Blum, Joel D; Chen, Celia Y et al. (2014) Mercury isotope study of sources and exposure pathways of methylmercury in estuarine food webs in the Northeastern U.S. Environ Sci Technol 48:10089-97
Pan, Qinxin; Hu, Ting; Malley, James D et al. (2014) A system-level pathway-phenotype association analysis using synthetic feature random forest. Genet Epidemiol 38:209-19
Wyszynski, Asaf; Tanyos, Sam A; Rees, Judy R et al. (2014) Body mass and smoking are modifiable risk factors for recurrent bladder cancer. Cancer 120:408-14
Norton, Gareth J; Douglas, Alex; Lahner, Brett et al. (2014) Genome wide association mapping of grain arsenic, copper, molybdenum and zinc in rice (Oryza sativa L.) grown at four international field sites. PLoS One 9:e89685

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