Fluorescent reagents based on conjugate polymers have proven useful for inexpensive and field-portable detection of nanogram to femtogram levels of nitroaromatic explosives, which have been commercialized. Conjugated polymers are advantageous in such sensing applications, because delocalization of their excited state exciton along the polymer chain provides sensor amplification and remarkable sensitivity. This technology will be adapted to design tests for separating and detecting toxicants, such as polycyclic aromatic hydrocarbons, found at Superfund sites. Fluorescent polymer reagents will be end-functionalized so they can be covalently anchored to chromatographic supports in order to permit separation of mixtures. This new technology will be developed with the aim of an inexpensive kit for the rapid simultaneous separation and identification of polycyclic aromatic organic Superfund toxicants that exhibit carcinogenic and/or endocrine disrupting properties. New fluorometric reagents will also be developed to separate and identify arsenate, cadmium(ll), lead(ll), mercury(ll), and chromium(VI), which are common Superfund inorganic toxicants found in contaminated sites. Special emphasis in the proposed work centers on specific toxicants that are being examined by other investigators in the center. The most promising analyses will be multiplexed on larger chromatographic substrates for quantification using microplate imaging methods. New fluorescent reagents developed for these tests are promising in other center applications, such as visualizing and localizing toxicants within whole organisms and cells. Thin-layer-chromatographic fluorescent assays that are low cost and readily portable could also be adapted to other applications, such as screening for hyperaccumulating plants, which are being examined in the UCSD center and are interesting because of their potential in remediation of arsenic soil contamination.

Public Health Relevance

The development of new technology that could be adapted for use in an inexpensive mobile kit for the detection of polycyclic aromatic hydrocarbons, dioxins, PCBs and PBBs, as well as the inorganic toxicants arsenic( /), chromium(Vl), lead(ll), cadmium(ll), and Hg(ll) encompass key classes of organic and inorganic Superfund toxicants known to pose cancer or endocrine disruption risks.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Hazardous Substances Basic Research Grants Program (NIEHS) (P42)
Project #
2P42ES010337-11A1
Application #
8263110
Study Section
Special Emphasis Panel (ZES1-JAB-J (SF))
Project Start
Project End
Budget Start
2012-04-26
Budget End
2013-03-31
Support Year
11
Fiscal Year
2012
Total Cost
$131,644
Indirect Cost
$46,644
Name
University of California San Diego
Department
Type
DUNS #
804355790
City
La Jolla
State
CA
Country
United States
Zip Code
92093
Fan, Weiwei; Evans, Ronald M (2017) Exercise Mimetics: Impact on Health and Performance. Cell Metab 25:242-247
Chen, Shujuan; Lu, Wenqi; Yueh, Mei-Fei et al. (2017) Intestinal NCoR1, a regulator of epithelial cell maturation, controls neonatal hyperbilirubinemia. Proc Natl Acad Sci U S A 114:E1432-E1440
Lu, Wenqi; Rettenmeier, Eva; Paszek, Miles et al. (2017) Crypt Organoid Culture as an in Vitro Model in Drug Metabolism and Cytotoxicity Studies. Drug Metab Dispos 45:748-754
Reinders, Megan E; Wardi, Gabriel; Bettencourt, Ricki et al. (2017) Increased Risk of Death, in the Hospital and Outside the Intensive Care Unit, for Patients With Cirrhosis After Cardiac Arrest. Clin Gastroenterol Hepatol 15:1808-1810
Liu, Yuanli; Yu, Chuanbai; Cao, Zhixin et al. (2017) A Highly Sensitive Enzymatic Catalysis System for Trace Detection of Arsenic in Water. Chemistry 23:10289-10292
Vollmann, Elisabeth H; Cao, Lizhi; Amatucci, Aldo et al. (2017) Identification of Novel Fibrosis Modifiers by In Vivo siRNA Silencing. Mol Ther Nucleic Acids 7:314-323
He, Nanhai; Fan, Weiwei; Henriquez, Brian et al. (2017) Metabolic control of regulatory T cell (Treg) survival and function by Lkb1. Proc Natl Acad Sci U S A 114:12542-12547
Shalapour, Shabnam; Lin, Xue-Jia; Bastian, Ingmar N et al. (2017) Inflammation-induced IgA+ cells dismantle anti-liver cancer immunity. Nature 551:340-345
Yoda, Emiko; Paszek, Miles; Konopnicki, Camille et al. (2017) Isothiocyanates induce UGT1A1 in humanized UGT1 mice in a CAR dependent fashion that is highly dependent upon oxidative stress. Sci Rep 7:46489
Koyama, Yukinori; Wang, Ping; Liang, Shuang et al. (2017) Mesothelin/mucin 16 signaling in activated portal fibroblasts regulates cholestatic liver fibrosis. J Clin Invest 127:1254-1270

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