The Analytical Chemistry Core (ACC;Core B) will be a new addition to Duke University's established Superfund Research Center (SRC). The mission of Duke's SRC has been and continues to be identifying the expression and mechanisms of developmental effects from early life exposure to Superfund chemicals and their transformation products. Addition of an analytical chemistry core to this center will enhance the biomedical and non-biomedical projects by providing state-of-the-art analytical support to monitor and quantify organic contaminant levels which can aid in identifying mechanisms of developmental toxicity. Adding the support of an ACC will provide the means of evaluating key relationships between exposures and body burdens, which will help determine what the potential biological """"""""costs"""""""" of early life exposures are for both humans and ecosystems;and furthermore, to determine whether or not remediation strategies are effective or if they increase these costs. The ACC will provide services for routine analyses of samples for organophosphate pesticides (e.g. chlorpyrifos), polycyclic aromatic hydrocarbons (PAHs), and brominated flame retardants (e.g. polybrominated diphenyl ethers) levels. In addition this core will assist in the identification of contaminant degradation products and/or metabolites which will be examined in several research projects. Lastly, the ACC will also serve as a teaching and training center for Duke University undergraduate and graduate students. The ACC will be supervised by Dr. P. Lee Ferguson, an Associate Professor of Environmental Science &Engineering with over ten years of experience in environmental mass spectrometry and trace analysis. Dr. Heather M. Stapleton, an Assistant Professor of Environmental Science and experienced trace analytical chemist, will serve as co-principal investigator and help manage the ACC. Drs. Ferguson and Stapleton currently supervise research laboratories equipped for high through-put extraction and analysis of samples for trace organic chemicals using a combination of gas chromatography mass spectrometry (GC/MS), liquid chromatography tandem mass spectrometry (HPLC/MS-MS), and high resolution mass spectrometry (HPLC/Orbitrap MS).

Public Health Relevance

The function of this Analytical Chemistry Core (Core B) will be to provide routine sample analysis and monitoring of Superfund contaminants examined in individual biomedical and non-biomedical projects, and in identifying degradation products and/or metabolites of these Superfund contaminants. This service will be utilized by all projects detailed in Duke's Superfund Research Center.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Hazardous Substances Basic Research Grants Program (NIEHS) (P42)
Project #
5P42ES010356-13
Application #
8659457
Study Section
Special Emphasis Panel (ZES1-SET-V)
Project Start
Project End
Budget Start
2014-04-01
Budget End
2015-03-31
Support Year
13
Fiscal Year
2014
Total Cost
$281,019
Indirect Cost
$100,878
Name
Duke University
Department
Type
DUNS #
044387793
City
Durham
State
NC
Country
United States
Zip Code
27705
Raftery, Tara D; Jayasundara, Nishad; Di Giulio, Richard T (2017) A bioenergetics assay for studying the effects of environmental stressors on mitochondrial function in vivo in zebrafish larvae. Comp Biochem Physiol C Toxicol Pharmacol 192:23-32
Mu, Jingli; Chernick, Melissa; Dong, Wu et al. (2017) Early life co-exposures to a real-world PAH mixture and hypoxia result in later life and next generation consequences in medaka (Oryzias latipes). Aquat Toxicol 190:162-173
Slotkin, Theodore A; Skavicus, Samantha; Card, Jennifer et al. (2017) In vitro models reveal differences in the developmental neurotoxicity of an environmental polycylic aromatic hydrocarbon mixture compared to benzo[a]pyrene: Neuronotypic PC12 Cells and embryonic neural stem cells. Toxicology 377:49-56
Luz, Anthony L; Godebo, Tewodros R; Smith, Latasha L et al. (2017) Deficiencies in mitochondrial dynamics sensitize Caenorhabditis elegans to arsenite and other mitochondrial toxicants by reducing mitochondrial adaptability. Toxicology 387:81-94
Hartman, Jessica H; Kozal, Jordan S; Di Giulio, Richard T et al. (2017) Zebrafish have an ethanol-inducible hepatic 4-nitrophenol hydroxylase that is not CYP2E1-like. Environ Toxicol Pharmacol 54:142-145
Meyer, Joel N; Chan, Sherine S L (2017) Sources, mechanisms, and consequences of chemical-induced mitochondrial toxicity. Toxicology 391:2-4
Slotkin, Theodore A; Skavicus, Samantha; Seidler, Frederic J (2017) Diazinon and parathion diverge in their effects on development of noradrenergic systems. Brain Res Bull 130:268-273
Lindberg, C D; Jayasundara, N; Kozal, J S et al. (2017) Resistance to polycyclic aromatic hydrocarbon toxicity and associated bioenergetic consequences in a population of Fundulus heteroclitus. Ecotoxicology 26:435-448
Abreu-Villa├ža, Yael; Levin, Edward D (2017) Developmental neurotoxicity of succeeding generations of insecticides. Environ Int 99:55-77
Jayasundara, Nishad; Fernando, Pani W; Osterberg, Joshua S et al. (2017) Cost of Tolerance: Physiological Consequences of Evolved Resistance to Inhabit a Polluted Environment in Teleost Fish Fundulus heteroclitus. Environ Sci Technol 51:8763-8772

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