Cognitive dysfunction is one of the most prevalent persisting adverse effects reported in epidemiological studies of environmental toxicant exposure. Cognitive as well as emotional dysfunction have been found in children after developmental exposure to a wide variety of toxicants including pesticides, heavy metals and polycyclic and polyhalogenated hydrocarbons. Cognitive and emotional impairment are also seen in experimental animal models of developmental neurotoxic exposures, showing the cause-and-effect relationships between developmental toxicant exposure and persisting deficits in learning, memory, attention and anxiety. In our previous research in the Duke University Superfund Research Center (DUSRC) we have demonstrated the adverse effects of organophosphate (OP) pesticide exposures and persisting cognitive as well as emotional effects in rat models. These have been found to be related to OP induced disruptions of the transmitter systems acetylcholine (ACh) as well as the monoamines, dopamine (DA) and serotonin (5HT). These neurotransmitter systems have been shown in a wide variety of studies to play important roles in the neural bases of cognitive and emotional function. In the next phase of research with rats, we will determine how disruptions of ACh, DA and 5HT across different toxicant classes can impair cognitive and emotional function. We hypothesize that the persisting impacts of pesticides, flame retardants and polycyclic hydrocarbons on cognitive and emotional function involve an adverse outcome pathway converging on this neurotransmitter triad. Pharmacological challenges will determine causative relationships between ACh, DA and 5HT disruption and behavioral impairments. The neurochemical and pharmacological studies will inform our innovative development of therapeutic treatments of persistent cognitive and emotional deficits, directly helping people who have cognitive and emotional dysfunction due to early life neurotoxic exposure. We will complement the classic rat model with the innovative higher throughput zebrafish neurobehavioral model. We will determine conserved neurobehavioral pathways for the neurotoxic induction of cognitive and emotional impairment across toxicant classes. Showing that the ACh, DA and 5HT triad is conserved in the zebrafish model will establish a higher-throughput model to expand the number of chemical exposures that can be evaluated and related to mechanistic changes that underlie behavioral dysfunction. Understanding the neurotransmitter involvement in neurotoxicant induced cognitive and emotional dysfunction will not only provide a common basis for understanding the functional impairments caused by diverse toxicants, it also provides a basis for devising effective therapeutics for neurotoxic damage. This will bring generalized advances to the understanding of neurobehavioral toxicology based on underlying synaptic mechanisms rather than on the behavioral effects of individual chemicals. This multi-component biologically-based investigation progresses beyond classic adverse outcome pathways to an adverse outcome interacting systems approach.
Project 1, ?Cholinergic and Monoaminergic Mechanisms of Persistent Neurobehavioral Toxicity? will provide important mechanistic information concerning the common neurobiological mechanism by which developmental toxicant exposure can cause persisting impairments in cognition and emotional function. This will provide the foundation for our development of therapeutic treatments using the rat model to help people overcome the persisting behavioral impairments from early life neurotoxicity. The zebrafish model will help in the more efficient screening of chemical for neurobehavioral risk, helping future generations to avoid such impairment.
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|Slotkin, Theodore A; Ko, Ashley; Seidler, Frederic J (2018) Does growth impairment underlie the adverse effects of dexamethasone on development of noradrenergic systems? Toxicology 408:11-21|
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