Exposure to fine particles and ultrafine particles in contaminated Superfund soils or produced from thermal treatment of hazardous substances may present health hazards, such as the exacerbation of pulmonary and cardiovascular diseases. The program objective includes determining the involvement of particle-generated reactive oxygen species (ROS) and oxidative stress mechanisms in toxicities observed within target tissues. The Oxidative Stress Core will provide support for Non-biomedical Project 1 and Biomedical projects 2, 4 and 5. Project 1 will determine how the molecular pollutants react with surfaces to form environmentally-persistent free radicals (EPFR) and why they are stabilized. Critical to this effort is elucidating whether the particles themselves redox-cycle to generate ROS. The core will assist with measurements of ROS produced in particle systems in vitro. Project 5 will determine the role of cytochrome P450 in the initiation/exacerbation of particle-induced ROS production. The core will provide support for the measurements of ROS and lipid oxidation in lung microsomes isolated from UFP-treated rats. Projects 2 and 4 will assess the role of UFP in exacerbating ischemia-reperfusion injury in the heart, as well as chronic obstructive pulmonary disease, through mechanisms involving oxidative stress. The core will service these projects by conducting measurements of ROS production and oxidative stress. This proposal describes assays that will be conducted and expertise to be provided. We will utilize the most current technologies, but will also use well-established approaches to measuring the necessary endpoints. Importantly, the core will adapt to the changing needs of the projects and will develop new techniques as necessary. The core is well equipped with the necessary expertise and personnel, and all relevant instrumentation available in the core Leader's laboratory, including spectrophotometers, HPLC, and LC-MS/MS, will be made available to the core. The strengths of the core include the breadth of expertise of the Core Leader and personnel, the accessibility/cooperativity of the core personnel, and the strong collaboration already in place between the core and the described projects.

Public Health Relevance

Oxygen is critical to life. However, in the cell, it can be converted to reactive forms that damage tissues and induce/exacerbate diseases. This condition, oxidative stress, is implicated in cancer, cardiovascular disease and aging. The Oxidative Stress Core will determine the role of oxidative stress in the exacerbation of diseases following exposure to airborne pollutants generated during thermal processing of organic wastes.

National Institute of Health (NIH)
National Institute of Environmental Health Sciences (NIEHS)
Hazardous Substances Basic Research Grants Program (NIEHS) (P42)
Project #
Application #
Study Section
Special Emphasis Panel (ZES1-SET-V)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Louisiana State University A&M Col Baton Rouge
Baton Rouge
United States
Zip Code
Hijano, Diego R; Siefker, David T; Shrestha, Bishwas et al. (2018) Type I Interferon Potentiates IgA Immunity to Respiratory Syncytial Virus Infection During Infancy. Sci Rep 8:11034
Haywood, Benjamin J; White, John R; Cook, Robert L (2018) Investigation of an early season river flood pulse: Carbon cycling in a subtropical estuary. Sci Total Environ 635:867-877
Connick, J Patrick; Reed, James R; Backes, Wayne L (2018) Characterization of Interactions Among CYP1A2, CYP2B4, and NADPH-cytochrome P450 Reductase: Identification of Specific Protein Complexes. Drug Metab Dispos 46:197-203
Potter, Phillip M; Guan, Xia; Lomnicki, Slawomir M (2018) Synergy of iron and copper oxides in the catalytic formation of PCDD/Fs from 2-monochlorophenol. Chemosphere 203:96-103
Harmon, Ashlyn C; Hebert, Valeria Y; Cormier, Stephania A et al. (2018) Particulate matter containing environmentally persistent free radicals induces AhR-dependent cytokine and reactive oxygen species production in human bronchial epithelial cells. PLoS One 13:e0205412
Jaligama, Sridhar; Patel, Vivek S; Wang, Pingli et al. (2018) Radical containing combustion derived particulate matter enhance pulmonary Th17 inflammation via the aryl hydrocarbon receptor. Part Fibre Toxicol 15:20
Dugas, Tammy R (2018) Unraveling mechanisms of toxicant-induced oxidative stress in cardiovascular disease. Curr Opin Toxicol 7:1-8
Feld-Cook, Elisabeth E; Bovenkamp-Langlois, Lisa; Lomnicki, Slawo M (2017) Effect of Particulate Matter Mineral Composition on Environmentally Persistent Free Radical (EPFR) Formation. Environ Sci Technol 51:10396-10402
Chuang, Gin C; Xia, Huijing; Mahne, Sarah E et al. (2017) Environmentally Persistent Free Radicals Cause Apoptosis in HL-1 Cardiomyocytes. Cardiovasc Toxicol 17:140-149
Jaligama, Sridhar; Saravia, Jordy; You, Dahui et al. (2017) Regulatory T cells and IL10 suppress pulmonary host defense during early-life exposure to radical containing combustion derived ultrafine particulate matter. Respir Res 18:15

Showing the most recent 10 out of 108 publications