Project 5. Phytoremediation to Degrade Airborne PCB Congeners from Soil &Groundwater Sources The overall goal of Project 5 is to provide engineering research (non-biomedical) for the remediation of sites containing airborne PCB congeners which may expose humans. Specifically, it is to determine whether plants can provide In situ phytoremediation of PCB congeners from the air and other airborne sources like dredged sediments at the planned Confined Disposal Facility (CDF) in East Chicago, Indiana, near two schools. Thus the Project focuses on PCB congeners of higher volatility which are present in Chicago air and which display significant mass, toxicity or persistence in the environment. Plants can uptake PCB congeners from soil and soil-water, intercept semi-volatile congeners from the air onto the waxy cuticle of leaves and bark, and metabolize contaminants directly. In addition, plants stimulate rhizosphere bioremediaton of PCBs by providing the habitat, redox potential, and substrate necessary for degradation. The significance of this project is that, by studying further the genomic, proteomic, and metabolomic basis of PCB phytoremediation, it will provide the scientific basis for the development and application of land management strategies for intervention at contaminated waste sites, and to break the continuous cycling of PCBs in the atmosphere and subsequent exposure to humans.
Four specific aims comprise Project 5: ? Identify plant metabolites of selected PCB congeners (PCB-3, 11, 15, 28, 52, 77, 153) and the uptake/resolution/metabolism of chiral compounds (PCB-95, 136) using GC/MS and LC/MS/MS ? Mineralize PCB mixtures (in mesocosms and site plots) by varying redox conditions which microbially dechlorinates PCBs under anoxic conditions and oxidizes the biphenyl ring under aerobic conditions ? Analyze the proteomic response and toxicity to pure cultures of selected aerobic PCB degraders and identified anaerobic degraders exposed to PCBs and PCB metabolites ? Characterize PCB-induced changes on the soil microbial community at CDF sites and sediments using T-RFLP analysis and proteomic analysis The leading themes of the competitive renewal are to identify more completely the PCB metabolites, the biotransformation proteins involved, and to demonstrate complete mineralization of PCB congeners in the root zone of plants by using the latest techniques of metabolomics and proteomics.

Public Health Relevance

Project 5 provides engineering research (non-biomedical) to provide the scientific basis for land management strategies to remediate contaminated sites and to intervene in the exposure of humans to PCBs and their metabolites. It is integrated with Project 4 on identifying the atmospheric sources of PCBs, with Project 6 on characterizing exposures near the planned confined disposal facility, and with the Synthesis Core. Analytical Core, and Research Translation Core.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Hazardous Substances Basic Research Grants Program (NIEHS) (P42)
Project #
5P42ES013661-07
Application #
8377290
Study Section
Special Emphasis Panel (ZES1-LWJ-M)
Project Start
Project End
Budget Start
2012-04-01
Budget End
2013-03-31
Support Year
7
Fiscal Year
2012
Total Cost
$186,631
Indirect Cost
$53,252
Name
University of Iowa
Department
Type
DUNS #
062761671
City
Iowa City
State
IA
Country
United States
Zip Code
52242
Herkert, Nicholas J; Spak, Scott N; Smith, Austen et al. (2017) Calibration and evaluation of PUF-PAS sampling rates across the Global Atmospheric Passive Sampling (GAPS) network. Environ Sci Process Impacts :
Sethi, Sunjay; Keil, Kimberly P; Chen, Hao et al. (2017) Detection of 3,3'-Dichlorobiphenyl in Human Maternal Plasma and Its Effects on Axonal and Dendritic Growth in Primary Rat Neurons. Toxicol Sci 158:401-411
Kania-Korwel, Izabela; Wu, Xianai; Wang, Kai et al. (2017) Identification of lipidomic markers of chronic 3,3',4,4',5-pentachlorobiphenyl (PCB 126) exposure in the male rat liver. Toxicology 390:124-134
Holland, Erika B; Feng, Wei; Zheng, Jing et al. (2017) An Extended Structure-Activity Relationship of Nondioxin-Like PCBs Evaluates and Supports Modeling Predictions and Identifies Picomolar Potency of PCB 202 Towards Ryanodine Receptors. Toxicol Sci 155:170-181
Li, Xueshu; Parkin, Sean R; Lehmler, Hans-Joachim (2017) Absolute configuration of 2,2',3,3',6-pentachlorinatedbiphenyl (PCB 84) atropisomers. Environ Sci Pollut Res Int :
Qian, Jiajie; Jennings, Brandon; Cwiertny, David M et al. (2017) Emerging investigator series: development and application of polymeric electrospun nanofiber mats as equilibrium-passive sampler media for organic compounds. Environ Sci Process Impacts 19:1445-1456
Gourronc, Francoise A; Robertson, Larry W; Klingelhutz, Aloysius J (2017) A delayed proinflammatory response of human preadipocytes to PCB126 is dependent on the aryl hydrocarbon receptor. Environ Sci Pollut Res Int :
Schroer, Hunter W; Langenfeld, Kathryn L; Li, Xueshu et al. (2017) Biotransformation of 2,4-dinitroanisole by a fungal Penicillium sp. Biodegradation 28:95-109
Marek, Rachel F; Thorne, Peter S; Herkert, Nicholas J et al. (2017) Airborne PCBs and OH-PCBs Inside and Outside Urban and Rural U.S. Schools. Environ Sci Technol 51:7853-7860
Dhakal, Kiran; Gadupudi, Gopi S; Lehmler, Hans-Joachim et al. (2017) Sources and toxicities of phenolic polychlorinated biphenyls (OH-PCBs). Environ Sci Pollut Res Int :

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