The Synthesis Core directly supports the research function of the University of lowa SBRP research program (isbrp) by providing PCB exposure mixtures as well as individual PCB congeners and PCB metabolites.
Specific Aim 1 is the maintenance of PCB mixtures and the preparation/maintenance of analytical standards. The Synthesis Core will serve as a repository for technical PCB mixtures (e.g., Aroclors 1242 and 1254) and a synthetic PCB mixture that approximates the average PCB profile found in Chicago air. In addition, the Synthesis Core will maintain analytical standards that are developed in close collaboration with the Analytical Core from pure PCB/PCB metabolites.
Specific Aim 2 is the synthesis of PCB congeners for use with the in vitro and in vivo studies of Research Projects #1, #2 and # 5. These PCB congeners will also be available to the Analytical Core for the preparation of analytical and quality control/assurance standards.
Specific Aim 3 is the synthesis of methoxylated and hydroxylated PCB metabolites. Hydroxylated PCB metabolites will be used by Projects #1, #2, #3, and #5 for in vitro and in vivo studies. Both the hydroxylated and methoxylated PCB derivatives will also be provided to the Analytical Core as analytical standards for the identification and quantification of (unknown) metabolites.
Specific Aim 4 encompasses the synthesis of miscellaneous compounds for the isbrp, including PCB quinones (Projects #1, #2 and #3), PCB glutathione/N-acetyl cysteine conjugates (Project #1), PCB immunogens (Project #1) and PCB sulfates (Project #3). The Synthesis Core will also provide diazomethane as derivatization agent as part of this Aim. This effort indirectly supports Projects #1, #2, #4, #5 and #6. All compounds will be synthesized, if necessary in large (gram) quantities, with straightforward synthetic approaches that are well established at the University of lowa. Drawing on their extensive experience with the synthesis of organohalogen compounds. Synthesis Core staff will be able to synthesize every compound of interest to the individual Research Projects.
|Wu, Xianai; Yang, Jun; Morisseau, Christophe et al. (2016) 3,3',4,4',5-Pentachlorobiphenyl (PCB 126) Decreases Hepatic and Systemic Ratios of Epoxide to Diol Metabolites of Unsaturated Fatty Acids in Male Rats. Toxicol Sci 152:309-22|
|Martinez, Andres; Schnoebelen, Douglas J; Hornbuckle, Keri C (2016) Polychlorinated biphenyl congeners in sediment cores from the Upper Mississippi River. Chemosphere 144:1943-9|
|Xin, Xing; Senthilkumar, P K; Schnoor, Jerald L et al. (2016) Effects of PCB126 and PCB153 on telomerase activity and telomere length in undifferentiated and differentiated HL-60 cells. Environ Sci Pollut Res Int 23:2173-85|
|Wangpradit, Orarat; Adamcakova-Dodd, Andrea; Heitz, Katharina et al. (2016) PAMAM dendrimers as nano carriers to investigate inflammatory responses induced by pulmonary exposure of PCB metabolites in Sprague-Dawley rats. Environ Sci Pollut Res Int 23:2128-37|
|Koh, Wen Xin; Hornbuckle, Keri C; Wang, Kai et al. (2016) Serum polychlorinated biphenyls and their hydroxylated metabolites are associated with demographic and behavioral factors in children and mothers. Environ Int 94:538-45|
|Osterberg, David; Scammell, Madeleine Kangsen (2016) PCBs in schools--where communities and science come together. Environ Sci Pollut Res Int 23:1998-2002|
|Herkert, Nicholas J; Martinez, Andres; Hornbuckle, Keri C (2016) A Model Using Local Weather Data to Determine the Effective Sampling Volume for PCB Congeners Collected on Passive Air Samplers. Environ Sci Technol 50:6690-7|
|Li, Miao; Teesch, Lynn M; Murry, Daryl J et al. (2016) Cytochrome c adducts with PCB quinoid metabolites. Environ Sci Pollut Res Int 23:2148-59|
|Wangpradit, Orarat; Rahaman, Asif; Mariappan, S V Santhana et al. (2016) Breaking the dogma: PCB-derived semiquinone free radicals do not form covalent adducts with DNA, GSH, and amino acids. Environ Sci Pollut Res Int 23:2138-47|
|Flor, Susanne; He, Xianran; Lehmler, Hans-Joachim et al. (2016) Estrogenicity and androgenicity screening of PCB sulfate monoesters in human breast cancer MCF-7 cells. Environ Sci Pollut Res Int 23:2186-200|
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