Inhalation exposure to semi-volatile PCBs in industrial cities, near the Great Lakes, in areas where dredging is occurring and at remediation sites has the potential to cause neurological, reproductive, and immunological toxicity in humans and animals. The Inhalation Toxicology Core was established with the primary goal to serve the projects and other research support cores with state-of-the-art animal facilities and rigorously controlled inhalation exposures to realistic mixtures of airborne PCB congeners.
The specific aims of this Core are:
Aim 1) Generate controlled PCB atmospheres in exposure chambers and collect airborne PCBs resulting from vaporization of common industrial PCB mixtures (supports Projects 1, 2, 3, 5 and the Analytical and Synthesis Cores);
Aim 2) Perform acute, subacute and subchronic inhalation exposures of laboratory animals to defined mixtures of atmospheric PCBs and to individual congeners, (supports Projects 1 and 2);
Aim 3) Provide a rodent vivarium for experimental animals used for the isbrp and execute a quality assurance program to monitor their health and welfare (supports Projects 1, 2, and 3);
Aim 4) Perform necropies on exposed, control and sentinel rodents and distribute tissues to project investigators in the isbrp (supports Projects 1,2, and 3). The Inhalation Toxicology Core exists within the Pulmonary Toxicology Facility (PTF) of the Environmental Health Sciences Research Center and consists of 6,000 ft2 of contiguous laboratories and offices. The PTF is nationally recognized for innovative animal models and exposure delivery systems. A full array of inhalation toxicology, aerosol science, and bioassay services are rendered. The Inhalation Toxicology Core is well equipped for generating and quantifying PCB vapors or aerosols;for performing nose-only inhalation exposures of rats and mice in acute, subacute or subchronic protocols;and for determining the degree of exposure and response. A new aim in this renewal is:
Aim 5) Perform airborne exposures to primary alveolar and airway epithelia and epithelial cell lines in Transwells with an apical air interface using vapor phase and particle bound PCB congeners. This will bring to the program a new approach for studying toxic effects of airborne PCBs on epithelial cells.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Hazardous Substances Basic Research Grants Program (NIEHS) (P42)
Project #
5P42ES013661-08
Application #
8451615
Study Section
Special Emphasis Panel (ZES1-LWJ-M)
Project Start
Project End
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
8
Fiscal Year
2013
Total Cost
$124,747
Indirect Cost
$45,548
Name
University of Iowa
Department
Type
DUNS #
062761671
City
Iowa City
State
IA
Country
United States
Zip Code
52242
Uwimana, Eric; Li, Xueshu; Lehmler, Hans-Joachim (2018) Human Liver Microsomes Atropselectively Metabolize 2,2',3,4',6-Pentachlorobiphenyl (PCB 91) to a 1,2-Shift Product as the Major Metabolite. Environ Sci Technol 52:6000-6008
Herkert, Nicholas J; Hornbuckle, Keri C (2018) Effects of room airflow on accurate determination of PUF-PAS sampling rates in the indoor environment. Environ Sci Process Impacts 20:757-766
Herkert, Nicholas J; Spak, Scott N; Smith, Austen et al. (2018) Calibration and evaluation of PUF-PAS sampling rates across the Global Atmospheric Passive Sampling (GAPS) network. Environ Sci Process Impacts 20:210-219
Dhakal, Kiran; Gadupudi, Gopi S; Lehmler, Hans-Joachim et al. (2018) Sources and toxicities of phenolic polychlorinated biphenyls (OH-PCBs). Environ Sci Pollut Res Int 25:16277-16290
Enayah, Sabah H; Vanle, Brigitte C; Fuortes, Laurence J et al. (2018) PCB95 and PCB153 change dopamine levels and turn-over in PC12 cells. Toxicology 394:93-101
Klinefelter, Kelsey; Hooven, Molly Kromme; Bates, Chloe et al. (2018) Genetic differences in the aryl hydrocarbon receptor and CYP1A2 affect sensitivity to developmental polychlorinated biphenyl exposure in mice: relevance to studies of human neurological disorders. Mamm Genome 29:112-127
Gourronc, Francoise A; Robertson, Larry W; Klingelhutz, Aloysius J (2018) A delayed proinflammatory response of human preadipocytes to PCB126 is dependent on the aryl hydrocarbon receptor. Environ Sci Pollut Res Int 25:16481-16492
Alam, Sinthia; Carter, Gwendolyn S; Krager, Kimberly J et al. (2018) PCB11 Metabolite, 3,3'-Dichlorobiphenyl-4-ol, Exposure Alters the Expression of Genes Governing Fatty Acid Metabolism in the Absence of Functional Sirtuin 3: Examining the Contribution of MnSOD. Antioxidants (Basel) 7:
Li, Xueshu; Holland, Erika B; Feng, Wei et al. (2018) Authentication of synthetic environmental contaminants and their (bio)transformation products in toxicology: polychlorinated biphenyls as an example. Environ Sci Pollut Res Int 25:16508-16521
Sethi, Sunjay; Keil, Kimberly P; Lein, Pamela J (2018) 3,3'-Dichlorobiphenyl (PCB 11) promotes dendritic arborization in primary rat cortical neurons via a CREB-dependent mechanism. Arch Toxicol 92:3337-3345

Showing the most recent 10 out of 298 publications