The Synthesis Core directly supports the research function of the University of lowa SBRP research program (isbrp) by providing PCB exposure mixtures as well as individual PCB congeners and PCB metabolites.
Specific Aim 1 is the maintenance of PCB mixtures and the preparation/maintenance of analytical standards. The Synthesis Core will serve as a repository for technical PCB mixtures (e.g., Aroclors 1242 and 1254) and a synthetic PCB mixture that approximates the average PCB profile found in Chicago air. In addition, the Synthesis Core will maintain analytical standards that are developed in close collaboration with the Analytical Core from pure PCB/PCB metabolites.
Specific Aim 2 is the synthesis of PCB congeners for use with the in vitro and in vivo studies of Research Projects #1, #2 and # 5. These PCB congeners will also be available to the Analytical Core for the preparation of analytical and quality control/assurance standards.
Specific Aim 3 is the synthesis of methoxylated and hydroxylated PCB metabolites. Hydroxylated PCB metabolites will be used by Projects #1, #2, #3, and #5 for in vitro and in vivo studies. Both the hydroxylated and methoxylated PCB derivatives will also be provided to the Analytical Core as analytical standards for the identification and quantification of (unknown) metabolites.
Specific Aim 4 encompasses the synthesis of miscellaneous compounds for the isbrp, including PCB quinones (Projects #1, #2 and #3), PCB glutathione/N-acetyl cysteine conjugates (Project #1), PCB immunogens (Project #1) and PCB sulfates (Project #3). The Synthesis Core will also provide diazomethane as derivatization agent as part of this Aim. This effort indirectly supports Projects #1, #2, #4, #5 and #6. All compounds will be synthesized, if necessary in large (gram) quantities, with straightforward synthetic approaches that are well established at the University of lowa. Drawing on their extensive experience with the synthesis of organohalogen compounds. Synthesis Core staff will be able to synthesize every compound of interest to the individual Research Projects.
|Liang, Yi; Meggo, Richard; Hu, Dingfei et al. (2014) Enhanced Polychlorinated Biphenyl Removal in a Switchgrass Rhizosphere by Bioaugmentation with Burkholderia xenovorans LB400. Ecol Eng 71:215-222|
|Yang, Shupeng; Shi, Weimin; Hu, Dingfei et al. (2014) In vitro and in vivo metabolite profiling of valnemulin using ultraperformance liquid chromatography-quadrupole/time-of-flight hybrid mass spectrometry. J Agric Food Chem 62:9201-10|
|Salman, Kadhim N; Stuart, Mary A; Schmidt, Jack et al. (2014) The effects of 3,3',4,4'-tetrabromobiphenyl on rats fed diets containing a constant level of copper and varying levels of molybdenum. Environ Sci Pollut Res Int 21:6400-9|
|Tehrani, Rouzbeh; Lyv, Monica M; Van Aken, Benoit (2014) Transformation of hydroxylated derivatives of 2,5-dichlorobiphenyl and 2,4,6-trichlorobiphenyl by Burkholderia xenovorans LB400. Environ Sci Pollut Res Int 21:6346-53|
|Vorrink, Sabine U; Severson, Paul L; Kulak, Mikhail V et al. (2014) Hypoxia perturbs aryl hydrocarbon receptor signaling and CYP1A1 expression induced by PCB 126 in human skin and liver-derived cell lines. Toxicol Appl Pharmacol 274:408-16|
|Ekuase, Edugie J; Lehmler, Hans-Joachim; Robertson, Larry W et al. (2014) Binding interactions of hydroxylated polychlorinated biphenyls (OHPCBs) with human hydroxysteroid sulfotransferase hSULT2A1. Chem Biol Interact 212:56-64|
|Wu, Xianai; Kammerer, Austin; Lehmler, Hans-Joachim (2014) Microsomal oxidation of 2,2',3,3',6,6'-hexachlorobiphenyl (PCB 136) results in species-dependent chiral signatures of the hydroxylated metabolites. Environ Sci Technol 48:2436-44|
|Shen, Hua; Li, Miao; Wang, Bingxuan et al. (2014) Dietary antioxidants (selenium and N-acetylcysteine) modulate paraoxonase 1 (PON1) in PCB 126-exposed rats. Environ Sci Pollut Res Int 21:6384-99|
|Hu, Xin; Adamcakova-Dodd, Andrea; Thorne, Peter S (2014) The fate of inhaled (14)C-labeled PCB11 and its metabolites in vivo. Environ Int 63:92-100|
|Sueyoshi, Tatsuya; Li, Linhao; Wang, Hongbing et al. (2014) Flame retardant BDE-47 effectively activates nuclear receptor CAR in human primary hepatocytes. Toxicol Sci 137:292-302|
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