The Superfund Research Program at the University of Iowa (isrp) investigates the consequences of atmospheric sources and exposures to semi-volatile, lower-chlorinated polychlorinated biphenyls (PCBs). The biomedical and environmental research projects of the isrp need a variety of study compounds ranging from technical and synthetic PCB mixtures to pure PCB congeners and their metabolites for studies of the volatilization, transport and exposure of these PCB congeners. A major obstacle for these studies is the large number of 209 PCB congeners plus the large number of 837 possible hydroxylated (OH-PCBs), sulfated (PCB sulfates), glucuronidated (PCB glucuronides) and other PCB metabolites. Many of these PCB derivatives are not available from commercial sources or have never been synthesized before. Moreover, obtaining PCB mixtures or individual PCB congeners from commercial sources can be expensive, especially if large quantities are needed for animal studies. Therefore, the MAJOR OBJECTIVE of the Synthesis Core is to provide a large variety of fully characterized and highly pure PCB derivatives to individual research projects and research support cores within the isrp. If time and resources allow, the Synthesis Core will also make study compound available to other researchers, especially researchers from other Superfund Research Programs. At the same time, the Synthesis Core will continue to develop novel synthetic strategies for the synthesis of PCB congeners and PCB metabolites as needed by isrp researchers.
The Specific Aims of the Synthesis Core are to 1) maintain and prepare PCB mixtures and pure PCB congeners; 2) prepare hydroxylated PCB derivatives; synthesize PCB sulfate metabolites; and 4) prepare miscellaneous compounds, including OH-PCB glucuronides and diazomethane. Synthesis Core researchers have extensive experience with the preparation, purification and characterization of all compounds requested by isrp research projects. The timely availability of PCB mixtures, individual OH-PCBs, PCB sulfates and other PCB metabolites will greatly enhance and, if the respective PCB metabolites are unavailable, enable the innovative studies proposed by isrp research projects.

Public Health Relevance

The Iowa Superfund Research Program (isrp) addresses sources, exposures and toxicity issues related to airborne polychlorinated biphenyls (PCBs), through determination of sources and magnitudes of exposure, studies on mechanisms of toxic responses, and community-based participatory research that addresses body burdens of parent and metabolic products of atmospheric PCBs in adults and school children. Studies test the feasibility of using plants to degrade PCBs and remediate contaminated areas in an actual PCB-regulated site, while the results of these and all laboratory and field studies are made available to all stakeholders. The efforts of the Synthesis Core in providing standards and study compounds provide critical support for these efforts.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Hazardous Substances Basic Research Grants Program (NIEHS) (P42)
Project #
2P42ES013661-10
Application #
8919609
Study Section
Special Emphasis Panel (ZES1-LWJ-J (SF))
Project Start
Project End
Budget Start
2015-09-30
Budget End
2016-03-31
Support Year
10
Fiscal Year
2015
Total Cost
$162,428
Indirect Cost
$54,860
Name
University of Iowa
Department
Type
DUNS #
062761671
City
Iowa City
State
IA
Country
United States
Zip Code
52246
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Enayah, Sabah H; Vanle, Brigitte C; Fuortes, Laurence J et al. (2018) PCB95 and PCB153 change dopamine levels and turn-over in PC12 cells. Toxicology 394:93-101
Klinefelter, Kelsey; Hooven, Molly Kromme; Bates, Chloe et al. (2018) Genetic differences in the aryl hydrocarbon receptor and CYP1A2 affect sensitivity to developmental polychlorinated biphenyl exposure in mice: relevance to studies of human neurological disorders. Mamm Genome 29:112-127

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