The research and regulatory communities acknowledge the need to develop analytical methods for PAHs and substitute PAHs (x-PAHs). The recent US EPA IRIS 2010 document Development of a Relative Potency Factory (RFP) Approach for Polycyclic Aromatic Hydrocarbon Mixtures says, "While whole mixture assessment is preferred . . . chemical analysis of the components of mixtures is limited . . . The RPF approach is limited by the small number of PAHs for which there are analytical chemistry." It is challenging to analyze PAHs and x-PAHs in environmental and biological matrices because the samples are complex, the numerous isomers of alkylated and high-molecular-mass compounds are often difficult to differentiate, and we often lack standards and suitable reference materials. We will provide rapid chemical screening and quantitative analysis of mixtures from applicable matrices. The Chemistry Core provides Superfund Center Investigators with critical specialized analytical expertise. We will develop advanced techniques and methods for PAHs and mixtures. The need for new analytical methods continues to top the list of data gaps cited by agency workgroups. We need to further develop our 1,201 analyte screening method to "know thy environment" paralling the genome "know thyself technical advances. We will continue to develop a synthesis unit to make chemical standards that are not commercially available. We enable the work of our biomedical projects by synthesizing PAH metabolites. We plan to expand our synthesis component to include PAHs and substituted PAHs that are not currently commercially available. The lack of commercial standards constitutes a critical barrier to analytical method development and subsequent biomedical testing. We will continue to develop a centralized repository for exposure-based mixture reference materials, standards, and passive sampling devices. We propose to develop new Superfund reference materials, including bioavailable Superfund based materials and pre-and post-remediation extracts. We will make these well-characterized mixtures available to SRP investigators. We will also prepare and distribute passive sampling devices to SRP investigators and US EPA project managers for pilot investigations and evaluation.

Public Health Relevance

Further developing methods for mixtures that identify 1000's of chemical is critical to understand the complete exposure. Creation of non-commercially available standards and real-world reference materials supports chemical and biological Superfund advancements. By offering PSDs we will encourage further adoption of bio-analytical tools and techniques in risk characterization and also gain important feedback.

National Institute of Health (NIH)
National Institute of Environmental Health Sciences (NIEHS)
Hazardous Substances Basic Research Grants Program (NIEHS) (P42)
Project #
Application #
Study Section
Special Emphasis Panel (ZES1-LWJ-D (SF))
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Oregon State University
United States
Zip Code
Madeen, Erin; Corley, Richard A; Crowell, Susan et al. (2015) Human in Vivo Pharmacokinetics of [(14)C]Dibenzo[def,p]chrysene by Accelerator Mass Spectrometry Following Oral Microdosing. Chem Res Toxicol 28:126-34
O'Connell, Steven G; Kincl, Laurel D; Anderson, Kim A (2014) Silicone wristbands as personal passive samplers. Environ Sci Technol 48:3327-35
O'Connell, Steven G; McCartney, Melissa A; Paulik, L Blair et al. (2014) Improvements in pollutant monitoring: optimizing silicone for co-deployment with polyethylene passive sampling devices. Environ Pollut 193:71-8
Kile, Molly L; Coker, Eric S; Smit, Ellen et al. (2014) A cross-sectional study of the association between ventilation of gas stoves and chronic respiratory illness in U.S. children enrolled in NHANESIII. Environ Health 13:71
Crowell, S R; Hanson-Drury, S; Williams, D E et al. (2014) In vitro metabolism of benzo[a]pyrene and dibenzo[def,p]chrysene in rodent and human hepatic microsomes. Toxicol Lett 228:48-55
Jariyasopit, Narumol; Zimmermann, Kathryn; Schrlau, Jill et al. (2014) Heterogeneous reactions of particulate matter-bound PAHs and NPAHs with NO3/N2O5, OH radicals, and O3 under simulated long-range atmospheric transport conditions: reactivity and mutagenicity. Environ Sci Technol 48:10155-64
Forsberg, Norman D; O'Connell, Steven G; Allan, Sarah E et al. (2014) Passive sampling coupled to ultraviolet irradiation: a useful analytical approach for studying oxygenated polycyclic aromatic hydrocarbon formation in bioavailable mixtures. Environ Toxicol Chem 33:177-81
Hillwalker, Wendy E; Anderson, Kim A (2014) Bioaccessibility of metals in alloys: evaluation of three surrogate biofluids. Environ Pollut 185:52-8
Jariyasopit, Narumol; McIntosh, Melissa; Zimmermann, Kathryn et al. (2014) Novel nitro-PAH formation from heterogeneous reactions of PAHs with NO2, NO3/N2O5, and OH radicals: prediction, laboratory studies, and mutagenicity. Environ Sci Technol 48:412-9
Bugel, Sean M; Bonventre, Josephine A; White, Lori A et al. (2014) Chronic exposure of killifish to a highly polluted environment desensitizes estrogen-responsive reproductive and biomarker genes. Aquat Toxicol 152:222-31

Showing the most recent 10 out of 61 publications