The overall goal of the Molecular Biology Core of this Alcohol Research Center is to study the association between specific genes and various aspects of alcoholism, alcohol abuse and alcoholic organ complications. The Core has developed polymerase chain reaction (PCR)-based genotyping assays for the alcohol metabolizing enzymes, ADH2, ADH3, and ALDH2 and has determined the allele frequencies of these loci in various collaborative studies. We will continue to offer these genotyping services to investigators in order to determine if there are further relationships between these enzymes and responses to alcohol. A new focus of the core is to establish new genotyping methods for other alcohol metabolizing enzymes, endorphins, and the genes related to dopamine and serotonin synthesis, degradation and neurotransmission. The restriction fragment length polymorphism (RFLP) assay or PCR analysis of polymorphic repetitive DNA sequences will be employed on genes for which polymorphisms are known. If a new mutation in a gene of interest is found or reported, we will develop a PCR-based genotyping assay instead of the RFLP analysis because it requires less DNA and the assays are easier to interpret. For genes in which polymorphisms are not yet known, we will search for polymorphic CA repeats or alternatively, amplify exons and/or introns and screen for single strand conformation polymorphisms in order to develop genotyping assays.

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National Institute on Alcohol Abuse and Alcoholism (NIAAA)
Specialized Center (P50)
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Indiana University Bloomington
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McClintick, Jeanette N; McBride, William J; Bell, Richard L et al. (2018) Gene expression changes in the ventral hippocampus and medial prefrontal cortex of adolescent alcohol-preferring (P) rats following binge-like alcohol drinking. Alcohol 68:37-47
Houck, Christa A; Grahame, Nicholas J (2018) Acute drug effects on habitual and non-habitual responding in crossed high alcohol preferring mice. Psychopharmacology (Berl) 235:2167-2175
Ding, Zheng-Ming; Ingraham, Cynthia M; Hauser, Sheketha R et al. (2017) Reduced Levels of mGlu2 Receptors within the Prelimbic Cortex Are Not Associated with Elevated Glutamate Transmission or High Alcohol Drinking. Alcohol Clin Exp Res 41:1896-1906
Linsenbardt, David N; Smoker, Michael P; Janetsian-Fritz, Sarine S et al. (2017) Impulsivity in rodents with a genetic predisposition for excessive alcohol consumption is associated with a lack of a prospective strategy. Cogn Affect Behav Neurosci 17:235-251
Bell, Richard L; Hauser, Sheketha R; Liang, Tiebing et al. (2017) Rat animal models for screening medications to treat alcohol use disorders. Neuropharmacology 122:201-243
Öztürk, Nail Can; Resendiz, Marisol; Öztürk, Hakan et al. (2017) DNA Methylation program in normal and alcohol-induced thinning cortex. Alcohol 60:135-147
Luczak, Susan E; Wall, Tamara L (2016) Gambling problems and comorbidity with alcohol use disorders in Chinese-, Korean-, and White-American college students. Am J Addict 25:195-202
Ding, Zheng-Ming; Ingraham, Cynthia M; Rodd, Zachary A et al. (2016) Alcohol drinking increases the dopamine-stimulating effects of ethanol and reduces D2 auto-receptor and group II metabotropic glutamate receptor function within the posterior ventral tegmental area of alcohol preferring (P) rats. Neuropharmacology 109:41-48
Kasten, C R; Frazee, A M; Boehm 2nd, S L (2016) Developing a model of limited-access nicotine consumption in C57Bl/6J mice. Pharmacol Biochem Behav 148:28-37
Zhou, Feng C; Resendiz, Marisol; Lo, Chiao-Ling et al. (2016) Cell-Wide DNA De-Methylation and Re-Methylation of Purkinje Neurons in the Developing Cerebellum. PLoS One 11:e0162063

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