The CELL/MOLECULAR BIOLOGY CORE provides several of the MAIN and PILOT PROJECTS with state-of-the-art support for their proposals primarily in assessments of fresh (unfixed) specimens. The specific services provided by the CELL/MOLECULAR BIOLOGY CORE include (a) extraction of DMA,RNA, and protein from tissue samples, (b) genotyping, real-time quantitative RT-PCR, (c) Western immunoblotting, (d) cloning and synthesis of cRNA riboprobes, (e) performing radioactive and non-radioactive in situ hybridization, (f) phosphorimaging analysis, (g) HPLC-based neurochemical assays, and (h) laser microdissection. In addition to the methodoligcal expertise, the CORE provides advice and collaboration for the design, implementation, and analyses of cell and molecular biology studies. The CORE is directed by the capable oversight of Drs. Middleton and Vallano. Through careful consultation and experimental design, DEARC Pis will benefit from uniform application of the highest level of quality control. Moreover, the raw data generated by the CELL/MOLECULAR BIOLOGY CORE will be routinely added into the central DEARC database, so that any investigator can analyze them for specific effects and possible correlations with data generated from their studies. The CELL/MOLECULAR BIOLOGY CORE personnel will assist each of the Pi's in this regard, and will also work closely with the ANIMAL and NEUROANATOMY CORES to ensure that the correct tissue samples are being utilized in each assay according to the experimental designs.

Agency
National Institute of Health (NIH)
Institute
National Institute on Alcohol Abuse and Alcoholism (NIAAA)
Type
Specialized Center (P50)
Project #
5P50AA017823-05
Application #
8537113
Study Section
Special Emphasis Panel (ZAA1-BB)
Project Start
Project End
Budget Start
2013-09-01
Budget End
2014-08-31
Support Year
5
Fiscal Year
2013
Total Cost
$51,321
Indirect Cost
$14,452
Name
State University of NY, Binghamton
Department
Type
DUNS #
090189965
City
Binghamton
State
NY
Country
United States
Zip Code
13902
Mantella, Nicole M; Youngentob, Steven L (2014) Prenatal alcohol exposure increases postnatal acceptability of nicotine odor and taste in adolescent rats. PLoS One 9:e102255
Santerre, Jessica L; Gigante, Eduardo D; Landin, Justine D et al. (2014) Molecular and behavioral characterization of adolescent protein kinase C following high dose ethanol exposure. Psychopharmacology (Berl) 231:1809-20
Doremus-Fitzwater, Tamara L; Buck, Hollin M; Bordner, Kelly et al. (2014) Intoxication- and withdrawal-dependent expression of central and peripheral cytokines following initial ethanol exposure. Alcohol Clin Exp Res 38:2186-98
Gigante, Eduardo D; Santerre, Jessica L; Carter, Jenna M et al. (2014) Adolescent and adult rat cortical protein kinase A display divergent responses to acute ethanol exposure. Alcohol 48:463-70
Miranda-Morales, Roberto Sebastián; Nizhnikov, Michael E; Waters, Dustin H et al. (2014) New evidence of ethanol's anxiolytic properties in the infant rat. Alcohol 48:367-74
Fabio, Maria Carolina; Nizhnikov, Michael E; Spear, Norman E et al. (2014) Binge ethanol intoxication heightens subsequent ethanol intake in adolescent, but not adult, rats. Dev Psychobiol 56:574-83
Anderson, Rachel I; Morales, Melissa; Spear, Linda P et al. (2014) Pharmacological activation of kappa opioid receptors: aversive effects in adolescent and adult male rats. Psychopharmacology (Berl) 231:1687-93
Varlinskaya, Elena I; Mooney, Sandra M (2014) Acute exposure to ethanol on gestational day 15 affects social motivation of female offspring. Behav Brain Res 261:106-9
Nizhnikov, Michael E; Kozlov, Andrey P; Kramskaya, Tatiana A et al. (2014) Central effects of ethanol interact with endogenous mu-opioid activity to control isolation-induced analgesia in maternally separated infant rats. Behav Brain Res 260:119-30
Anderson, Rachel I; Spear, Linda P (2014) Age differences in ethanol discrimination: acquisition and ethanol dose generalization curves following multiple training conditions in adolescent and adult rats. Alcohol Clin Exp Res 38:186-94

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