The Neuropathology Sub-Core (NP) provides complete brain banking and neuropathology diagnostic services for the Pittsburgh ADRC with the goal of providing well-characterized tissues for research studies. Brain autopsies are performed on a 24 hour basis and the average post-mortem time over the past four years is ~6 hours. Over 150 frozen samples and CSF are banked. The brain is then fixed in 10% buffered formalin and 20 areas are taken for microscopic evaluation. Stains used include H&E, immunocytochemical (beta-A4 amyloid, tau, alpha-synuclein, and ubiquitin, TDP-43) and silver stains (Bielschowsky). Evaluation is performed using NIA-RI guidelines. Lewy Body pathology assessed per the DLB consensus criteria of McKeith et. al. Tissues and data are made available to investigators only after approval by the ADRC Executive Committee. During the last 5 years (2004-2008), the NP Sub-Core has evaluated a total of 289 cases: 115 of these were part of the ADRC cohort. Thirty-five of the 289 cases were part of ALS bank, 16 came from the Movement Disorders Clinic and 11 were from the Late Life Mood Disorder Cohort. 14 CJD cases (or "rule out CJD") were also evaluated. 98 other cases were evaluated and included gross-only evaluations for brains that were removed for other ADCs or brain banking groups, medical autopsies for possible controls, and cases associated with the ADRC who were not part of the Cohort. Postmortem times for ADRC cohort cases with frozen material banked remained low at 5.1 hrs average. The NP Sub-Core provides regular updates to the NACC NP Database. The Sub-Core is also an active participant in local, national and international conferences. The Sub-Core maintains a reference laboratory (including a Ar/Kr laser confocal microscope) for development of quantitative analyses. The Genetics Sub-Core maintains a repository of DNA and genotypes on all participants of the Pittsburgh ADRC.
The aims of the Genetics Sub-Core are: 1) to collect and bank DNA from blood and brain tissues from all participants in the Pittsburgh ADRC;2), to generate genotype data for the APOE polymorphisms in the coding (codons 28, 112, 158) and regulatory (-491 A/T, -427 T/C, -219 G/T, -186 G/T and +113 G/C) regions, and other new genetic risk markers associated with late-onset Alzheimer's disease from DNA obtained in Aim 1;and 3) to provide banked DNA and genotype data to the ADRC Registry Database and to ADRC affiliated R01 grants, pilot projects and NIA repositories so that the DNA can be used for other genetic markers screening and genotype data can be used to assess the genetic risk for Alzheimer's disease.

Public Health Relevance

The NP Sub-Core provides a final diagnosis for correlation with clinical behavior. It also banks tissues for use in research on Alzheimer's Disease. The Genetics Sub-Core provided critical information regarding the ApoE status of all members of the cohort.

National Institute of Health (NIH)
National Institute on Aging (NIA)
Specialized Center (P50)
Project #
Application #
Study Section
Special Emphasis Panel (ZAG1-ZIJ-4)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Pittsburgh
United States
Zip Code
Flatt, Jason D; Liptak, Amy; Oakley, Mary Ann et al. (2015) Subjective experiences of an art museum engagement activity for persons with early-stage Alzheimer's disease and their family caregivers. Am J Alzheimers Dis Other Demen 30:380-9
Wang, Xingbin; Lopez, Oscar L; Sweet, Robert A et al. (2015) Genetic determinants of disease progression in Alzheimer's disease. J Alzheimers Dis 43:649-55
Klunk, William E; Koeppe, Robert A; Price, Julie C et al. (2015) The Centiloid Project: standardizing quantitative amyloid plaque estimation by PET. Alzheimers Dement 11:1-15.e1-4
Hu, Wei; MacDonald, Matthew L; Elswick, Daniel E et al. (2015) The glutamate hypothesis of schizophrenia: evidence from human brain tissue studies. Ann N Y Acad Sci 1338:38-57
Riverol, Mario; Becker, James T; López, Oscar L et al. (2015) Relationship between Systemic and Cerebral Vascular Disease and Brain Structure Integrity in Normal Elderly Individuals. J Alzheimers Dis 44:319-28
Chaudhry, Mamoonah; Wang, Xingbin; Bamne, Mikhil N et al. (2015) Genetic variation in imprinted genes is associated with risk of late-onset Alzheimer's disease. J Alzheimers Dis 44:989-94
Sanders, Laurie H; McCoy, Jennifer; Hu, Xiaoping et al. (2014) Mitochondrial DNA damage: molecular marker of vulnerable nigral neurons in Parkinson's disease. Neurobiol Dis 70:214-23
Raji, Cyrus A; Erickson, Kirk I; Lopez, Oscar L et al. (2014) Regular fish consumption and age-related brain gray matter loss. Am J Prev Med 47:444-51
Hong, Young T; Veenith, Tonny; Dewar, Deborah et al. (2014) Amyloid imaging with carbon 11-labeled Pittsburgh compound B for traumatic brain injury. JAMA Neurol 71:23-31
Beecham, Gary W; Hamilton, Kara; Naj, Adam C et al. (2014) Genome-wide association meta-analysis of neuropathologic features of Alzheimer's disease and related dementias. PLoS Genet 10:e1004606

Showing the most recent 10 out of 361 publications