Filariae and Leishmania endemicity areas overlap in many regions of Mali. Both represent important public health problem due to their distribufion, prevalence, and associated clinical features. These 2 neglected parasific tropical diseases are vector borne with mosquitoes and phlebotomine sand flies as respective vectors for filariasis and leishmaniasis. In co endemic areas, the interacfion between filariae, sand fly saliva and Leishmania and how they modulate host immunity and whether such interacfions have an effect on disease outcome is of great importance, not well understood, and the aim of this project.
The aim of this study is first to address whether salivary proteins of a sand fly are capable of altering the funcfion of APC (anfigen presenfing cells), particularly the macrophages, and second how patent filariasis may alter the immune response of the human host to these proteins. The study will be done in villages of Mopfi and Segou Districts of Mali. An immune chromatographic card test (ICT) for circulafing filarial antigen using whole blood will be performed in these areas. For exposure to salivary proteins, the sera of both mf- and mf+ individuals will be then screened by Elisa and Western blot. For cutaneous leishmaniasis (CL), a leishmanin skin test (LST) will be conducted to identify a cohort of individuals that are LST negative at the outset of the study. This study will have a significant impact on the immune response of humans to Leishmania infecfions as a result of an altered immune response to salivary proteins. All the data generated will be novel and will contribute tremendously to the scientific community and our knowledge of the complexity of the immune response. Furthermore, establishing the effects of salivary proteins on human Mij) (which have not been addressed in the past) may lead to the discovery of molecules with adjuvant-like property that can be important in designing potential vaccines.

Public Health Relevance

This study has a public health significance as it will contribute to understand how the immune response can be affected in presence of 2 parasites. The study will provide informafion that will guide vaccine and/or adjuvant development to fight these two endemic diseases in poor countries.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Specialized Center (P50)
Project #
1P50AI098505-01
Application #
8304445
Study Section
Special Emphasis Panel (ZAI1-AWA-M (J1))
Project Start
Project End
2013-07-31
Budget Start
2012-08-01
Budget End
2013-07-31
Support Year
1
Fiscal Year
2012
Total Cost
$72,652
Indirect Cost
$5,382
Name
University of Bamako
Department
Type
DUNS #
565539819
City
Bamako
State
Country
Mali
Zip Code
BP E2-528
Touré, Mahamoudou; Petersen, Pelle T; Bathily, Sidy N'd et al. (2017) Molecular Evidence of Malaria and Zoonotic Diseases Among Rapid Diagnostic Test-Negative Febrile Patients in Low-Transmission Season, Mali. Am J Trop Med Hyg 96:335-337
Traoré, Bourama; Oliveira, Fabiano; Faye, Ousmane et al. (2017) Correction: Prevalence of Cutaneous Leishmaniasis in Districts of High and Low Endemicity in Mali. PLoS Negl Trop Dis 11:e0005379
Coulibaly, C A; Sissoko, I; Traore, B et al. (2016) Diversity of Sand Flies (Diptera: Psychodidae: Phlebotominae) in Two Different Eco-Climatic and Endemic Zones of Cutaneous Leishmaniasis in Mali, West Africa. J Med Entomol 53:923-927
Traoré, Bourama; Oliveira, Fabiano; Faye, Ousmane et al. (2016) Prevalence of Cutaneous Leishmaniasis in Districts of High and Low Endemicity in Mali. PLoS Negl Trop Dis 10:e0005141
H3Africa Consortium (see original citation for additional authors) (2014) Research capacity. Enabling the genomic revolution in Africa. Science 344:1346-8
Wei, Stanley C; Vanden Eng, Jodi L; Patterson, Amy E et al. (2012) Validity of Expanded Program on Immunization Contact Method health behavior estimates in Mali. J Infect Dis 205 Suppl 1:S112-9