This project is designed to establish novel treatments to decrease the risk of PTOA, and to investigate new diagnostic tools to identify patients at high risk of PTOA. The goal is to provide effective immediate preclinical information to support clinical application of these treatments and diagnostic tools. The treatments of interest are: 1) cytoprotective treatment that prevents immediate necrotic and acute apoptotic chondrocyte death at/near a site of acute cartilage injury, and 2) chondrocyte metabolic enhancement treatment developed to improve chondrocyte energy production, to restore anabolic function, and to suppress catabolic activities, by modulating the injury-related acute inflammatory response. These treatments will be piloted using a survival rabbit model. In this model, after controlled surgical insults that replicate major factors contributing to pathogenesis of human PTOA following joint injuries (i.e., acute joint injury and excessive cumulative mechanical stress to the injured cartilage), progressive cartilage loss predictably develops in the experimental joints (knees) in a relatively short period (8 weeks). Using this animal model of rapid-progression PTOA, work is proposed to test if the above treatments are capable of mitigating the early biologically mediated disease process of PTOA in vivo, and whether the treatments effectively decrease subsequent cartilage loss. The diagnostic tools of interest are: 1) MR imaging that visualizes/measures inflammatory anatomical changes and early intra-cartilage degenerative changes prior to cartilage loss, and 2) molecular biomarker analysis that measures whole-joint inflammation and whole-joint cartilage metabolic activity. The diagnostic power of these clinically applicable non- or minimally-invasive tools will be tested in a goat survival model of acute cartilage injury (created by means of precisely controlled blunt impaction insult). Work is proposed to test if these tools are capable of characterizing the severity of acute joint injury in vivo (particularly focusing on cartilage damage), and to test if subsequent progression of cartilage loss in these joints is reliably predictable using the early (<1 month) information provided by these diagnostic tools.
This project will provide preclinical evidence that supports promise of a novel acute treatment strategy to effectively ameliorate the disease process of PTOA, and it will demonstrate the capability of the diagnostic methods to identify patients at high risk of PTOA. These advancements will accelerate translational research to improve treatment of joint injuries, to decrease the risk of PTOA.
|Goetz, Jessica E; Coleman, Mitchell C; Fredericks, Douglas C et al. (2016) Time-dependent loss of mitochondrial function precedes progressive histologic cartilage degeneration in a rabbit meniscal destabilization model. J Orthop Res :|
|Ayati, Bruce P; Kapitanov, Georgi I; Coleman, Mitchell C et al. (2016) Mathematics as a conduit for translational research in post-traumatic osteoarthritis. J Orthop Res :|
|Zhou, C; Zheng, H; Buckwalter, J A et al. (2016) Enhanced phagocytic capacity endows chondrogenic progenitor cells with a novel scavenger function within injured cartilage. Osteoarthritis Cartilage 24:1648-55|
|Dibbern, Kevin; Kempton, Laurence B; Higgins, Thomas F et al. (2016) Fractures of the tibial plateau involve similar energies as the tibial pilon but greater articular surface involvement. J Orthop Res :|
|Anderson, Donald D; Long, Steven; Thomas, Geb W et al. (2016) Objective Structured Assessments of Technical Skills (OSATS) Does Not Assess the Quality of the Surgical Result Effectively. Clin Orthop Relat Res 474:874-81|
|Segal, Neil A; Frick, Eric; Duryea, Jeffrey et al. (2016) Comparison of tibiofemoral joint space width measurements from standing CT and fixed flexion radiography. J Orthop Res :|
|Kempton, Laurence B; Dibbern, Kevin; Anderson, Donald D et al. (2016) Objective Metric of Energy Absorbed in Tibial Plateau Fractures Corresponds Well to Clinician Assessment of Fracture Severity. J Orthop Trauma 30:551-6|
|Anderson, Donald D; Kilburg, Anthony T; Thomas, Thaddeus P et al. (2016) Expedited CT-Based Methods for Evaluating Fracture Severity to Assess Risk of Post-Traumatic Osteoarthritis After Articular Fractures. Iowa Orthop J 36:46-52|
|Martin, James A; Anderson, Donald D; Goetz, Jessica E et al. (2016) Complementary models reveal cellular responses to contact stresses that contribute to post-traumatic osteoarthritis. J Orthop Res :|
|Bonasia, Davide Edoardo; Martin, James A; Marmotti, Antonio et al. (2016) The use of autologous adult, allogenic juvenile, and combined juvenile-adult cartilage fragments for the repair of chondral defects. Knee Surg Sports Traumatol Arthrosc 24:3988-3996|
Showing the most recent 10 out of 82 publications