The overall goal of this Specialized Resource is to provide state-of-the-art high-resolution imaging, quantitative image analysis and modeling, surgery and pathology expertise to the main and developmental Projects. In addition, the Resource serves as an innovative facility to further develop and validate new cancer imaging approaches required in the different projects. Whole body and microscopic intravital imaging in live animals will be essential in establishing imaging-phenotype correlations, in validating targets and linked biomarkers, and in assessing efficacy of therapeutic agents. Surgical expertise is essential to all projects for chamber preparations, orthotopic tumor implantations, exteriorization of organs for imaging and physiologic monitoring during prolonged imaging sessions. Pathology services includes guidance and supervision in necropsy and tissue harvesting, tissue imaging, histopathologic review of tissues within pathophysiologic context and performing advanced immunohistochemistry. The Resource will continue to provide stability to the overall program and allow the investigators to focus on biological aspects and efficiently address critical issues relevant to the goals of this ICIMIC. Added benefits of this Resource are the high level of quality control, consistency of protocols and efficient cross-referencing of imaging results from different projects, high level maintenance of all equipment, 24/7 access to all imaging systems. The Resource also plays a critical role in training of junior staff (through the Career Development Program), fellows and graduate students.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Specialized Center (P50)
Project #
5P50CA086355-15
Application #
8693592
Study Section
Special Emphasis Panel (ZCA1-SRLB-9)
Project Start
Project End
Budget Start
2014-07-01
Budget End
2015-06-30
Support Year
15
Fiscal Year
2014
Total Cost
$77,684
Indirect Cost
Name
Massachusetts General Hospital
Department
Type
DUNS #
073130411
City
Boston
State
MA
Country
United States
Zip Code
02199
Keliher, Edmund J; Klubnick, Jenna A; Reiner, Thomas et al. (2014) Efficient acid-catalyzed (18) F/(19) F fluoride exchange of BODIPY dyes. ChemMedChem 9:1368-73
Dubach, J Matthew; Vinegoni, Claudio; Weissleder, Ralph (2014) Steady state anisotropy two-photon microscopy resolves multiple, spectrally similar fluorophores, enabling in vivo multilabel imaging. Opt Lett 39:4482-5
Pittet, Mikael J; Nahrendorf, Matthias; Swirski, Filip K (2014) The journey from stem cell to macrophage. Ann N Y Acad Sci 1319:1-18
Thurber, Greg M; Reiner, Thomas; Yang, Katherine S et al. (2014) Effect of small-molecule modification on single-cell pharmacokinetics of PARP inhibitors. Mol Cancer Ther 13:986-95
Courtis, Alexandra M; Santos, Sofia A; Guan, Yinghua et al. (2014) Monoalkoxy BODIPYs--a fluorophore class for bioimaging. Bioconjug Chem 25:1043-51
Hideshima, T; Mazitschek, R; Santo, L et al. (2014) Induction of differential apoptotic pathways in multiple myeloma cells by class-selective histone deacetylase inhibitors. Leukemia 28:457-60
Kim, Eunha; Yang, Katherine S; Giedt, Randy J et al. (2014) Red Si-rhodamine drug conjugates enable imaging in GFP cells. Chem Commun (Camb) 50:4504-7
Dubach, J M; Vinegoni, C; Mazitschek, R et al. (2014) In vivo imaging of specific drug-target binding at subcellular resolution. Nat Commun 5:3946
Turetsky, Anna; Kim, Eunha; Kohler, Rainer H et al. (2014) Single cell imaging of Bruton's tyrosine kinase using an irreversible inhibitor. Sci Rep 4:4782
Aceto, Nicola; Bardia, Aditya; Miyamoto, David T et al. (2014) Circulating tumor cell clusters are oligoclonal precursors of breast cancer metastasis. Cell 158:1110-22

Showing the most recent 10 out of 266 publications