The purpose of the Animal Models Core facility is to support and centralize certain aspects of the work ineach project proposed. It is our belief that prostate cancer biology is best studied in animal model systemsthat allow experimental monitoring of progression events such as metastasis and androgen-independentprogression. This facility brings together expertise in mouse genetics and human tumor xenografs that willallow basic molecular biological questions to be addressed in state-of-the art model systems with directrelevance to the clinical situation. It also offers an opportunity for translational research questions to beaddressed immediately in relevant pre-clinical models. The Core consists of six distinct components whichwill allow biologically based hypotheses to be tested in Suitable animal models or human prostate cancerxenografts.
The Specific Aims of this Core are as follows:1. To provide SCID mice to the five research projects2. To propagate four human prostate cancer xenografts: LAPC4, LAPC9, CWR22, and LUCAP on SCIDmice for Investigators in the SPORE.3. To maintain model mice, such as Pten and Cox-2 conditional knock-out, AKT and Myc transgenic miceas well as reporter lines needed for each project.4. To provide technical assistance for in vivo experiments, such as harvest xenografts, measure PSA level,and inject pro-drugs for pre-clinical trials.5. To provide expertise/consultation on animal experimentation and surgical techniques required for theproposed studies.6. To provide expertise/consultation for the generation of new models.
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