The goals of the Pathology Core are to collect, prioritize, distribute and interrogate both human and mouse models of prostate cancer. The mission of the tissue acquisition component of the Pathology Gore is to: provide a reliable and continuous source of well characterized and annotated prostate tissue samples for evaluation of tumor markers and other studies;minimize investigator efforts needed to obtain human tissues;Improve the quality of human tissues collected from donors after appropriate consent;assure that the tissues meet stated diagnoses;minimize the chances of a diagnostic specimen being compromised by improper handling;foster collaborative efforts between basic scientists and clinicians by providing hands on expertise with all aspects of tissue processing including histology, immunohistochemistry, imaging, etc.;and ensure fair distribution of tissue under the direction of the SPORE Executive Committee. The Core has also constructed microarrays from over 1000 cases of prostate cancer with clinical follow up, including normal and malignant tissues from patients of diverse ethnic backgrounds. The Core collects and annotates diverse specimens including fixed and frozen tumor tissue, tissue mircroarrays, whole mounts, paraffin blocks, fresh tissue serum, plasma, urine, DNA/RNA, and fixed tissues from animal xenografts and GEM models. Services include Veridex CTC enumeration, CTC enumeration and characterization in collaboration with the Tseng lab, quantitative immunohistochemistry, laser microdissection, FISH and double and single immunofluoresence. The Core works closely with clinical investigators to collect, process and examine critical biomarkers associated with those clinical trials. The core provides expertise with diagnosis of prostate neoplasia in biopsies and prostatectomy specimens as well as multiple murine models of prostate cancer. The Core works closely with other SPORE sites to standardize assays of common importance and works closely with the Statistics and Bioinformatics Cores linking tissues to the prostate clinical database and to national tissue banks (CaBIG). The Pathology Core is heavily invested in developing and acquiring new technologies to solve problems related to the procurement and analysis of all pathological specimens.

Public Health Relevance

The pathology core is an essential component of the projects, all of which have a translational arm requiring analysis of human and animal tissues. The Core makes available fresh, frozen, and fixed biopsy and prostatectomy tissues, provides DNA and RNA from cancer and normal tissues, and assists with performing immunohistochemistry, Veridex CTC enumeration, laser capture microscopy, whole slide scanning, and image analysis. The Core also is essential for collaborative research involving evaluation of murine models of prostate cancer.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Specialized Center (P50)
Project #
5P50CA092131-12
Application #
8760363
Study Section
Special Emphasis Panel (ZCA1-RPRB-M)
Project Start
Project End
Budget Start
2014-09-01
Budget End
2015-08-31
Support Year
12
Fiscal Year
2014
Total Cost
$179,158
Indirect Cost
$75,313
Name
University of California Los Angeles
Department
Type
DUNS #
092530369
City
Los Angeles
State
CA
Country
United States
Zip Code
90095
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Mitra, Mithun; Ho, Linda D; Coller, Hilary A (2018) An In Vitro Model of Cellular Quiescence in Primary Human Dermal Fibroblasts. Methods Mol Biol 1686:27-47
Li, Jiayun; Speier, William; Ho, King Chung et al. (2018) An EM-based semi-supervised deep learning approach for semantic segmentation of histopathological images from radical prostatectomies. Comput Med Imaging Graph 69:125-133
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Lee, Ha Neul; Mitra, Mithun; Bosompra, Oye et al. (2018) RECK isoforms have opposing effects on cell migration. Mol Biol Cell 29:1825-1838
Aggarwal, Rahul; Huang, Jiaoti; Alumkal, Joshi J et al. (2018) Clinical and Genomic Characterization of Treatment-Emergent Small-Cell Neuroendocrine Prostate Cancer: A Multi-institutional Prospective Study. J Clin Oncol 36:2492-2503

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