Center for the Neurobiology of Addiction Treatment Animal and Tissue Core Summary Dr. Mark Ferris, Core Director; Dr. Paul Czoty, co-investigator The overall objective of the Animal and Tissue Core is to generate, track and distribute tissue from experimental animals to multiple in vivo and in vitro experiments. The Core will centralize and standardize the generation, storage and transfer of brain tissue from rodents to Projects 2 and 3. These studies include self- administration, viral mediated gene knockdown of dopaminergic D2 receptors and acute/chronic treatment with putative medications identified in Project 1. By taking responsibility for the generation of these animals for studies in Projects 2 and 3, the Core will ensure that all subjects will be treated and euthanized in a standardized manner. Brain and peripheral tissue will be organized and stored by the Core, which will ensure accurate recording of the subjects' behavioral and pharmacological histories. The Core will distribute this tissue under blinded conditions to investigators in Projects 2 and 3 for imaging and biochemical studies. Careful preparation and handling of these samples and meticulous organization of information are required to ensure that reliable conclusions can be obtained from experiments using these tissue samples, which makes the Animal and Tissue Core an essential component of the Center. The specific goals are: 1. To generate groups of rats with viral mediated gene knockdown targeted against D2 receptors for Projects 2 and 3. 2. To generate groups of rats with a history of cocaine self-administration (with and without medication treatment and viral mediated gene knockdown) for Projects 2 and 3. 3. To provide a central locus for detailed record-keeping for all tissues stored within the Core and to provide timely distribution of this tissue under double-blinded conditions to Projects 2 and 3. 4. To collect and bank rodent brains that will be generated by cocaine self-administration studies in Project 1 for biochemical analyses of putative treatment drugs and other potential future uses. 5. To collect and bank nonhuman primate blood and cerebrospinal fluid (CSF) for analysis of metabolites of putative treatment drugs and other potential future uses, and bank nonhuman primate brain samples as they become available after studies in Project 1 are completed. 6. To maintain the tissue bank and periodically biopsy tissue to ensure tissue integrity.

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Specialized Center (P50)
Project #
5P50DA006634-25
Application #
9232103
Study Section
Special Emphasis Panel (ZDA1-NXR-B)
Project Start
Project End
Budget Start
2017-03-01
Budget End
2018-02-28
Support Year
25
Fiscal Year
2017
Total Cost
$116,491
Indirect Cost
$41,336
Name
Wake Forest University Health Sciences
Department
Type
Domestic Higher Education
DUNS #
937727907
City
Winston-Salem
State
NC
Country
United States
Zip Code
27157
Brodnik, Zachary D; Ferris, Mark John; Jones, Sara R et al. (2016) Reinforcing Doses of Intravenous Cocaine Produce Only Modest Dopamine Uptake Inhibition. ACS Chem Neurosci :
Eldeeb, Khalil; Leone-Kabler, Sandra; Howlett, Allyn C (2016) CB1 cannabinoid receptor-mediated increases in cyclic AMP accumulation are correlated with reduced Gi/o function. J Basic Clin Physiol Pharmacol 27:311-22
Porrino, Linda J; Beveridge, Thomas J R; Smith, Hilary R et al. (2016) Functional consequences of cocaine expectation: findings in a non-human primate model of cocaine self-administration. Addict Biol 21:519-29
Karkhanis, Anushree N; Beveridge, Thomas J R; Blough, Bruce E et al. (2016) The individual and combined effects of phenmetrazine and mgluR2/3 agonist LY379268 on the motivation to self-administer cocaine. Drug Alcohol Depend 166:51-60
McGinnis, Molly M; Siciliano, Cody A; Jones, Sara R (2016) Dopamine D3 autoreceptor inhibition enhances cocaine potency at the dopamine transporter. J Neurochem 138:821-9
Czoty, P W; Blough, B E; Fennell, T R et al. (2016) Attenuation of cocaine self-administration by chronic oral phendimetrazine in rhesus monkeys. Neuroscience 324:367-76
Porrino, Linda J; Miller, Mack D; Smith, Hilary R et al. (2016) Neural Correlates of Exposure to Cocaine Cues in Rhesus Monkeys: Modulation by the Dopamine Transporter. Biol Psychiatry :
Siciliano, Cody A; Fordahl, Steve C; Jones, Sara R (2016) Cocaine Self-Administration Produces Long-Lasting Alterations in Dopamine Transporter Responses to Cocaine. J Neurosci 36:7807-16
Gould, R W; Czoty, P W; Porrino, L J et al. (2016) Social Status in Monkeys: Effects of Social Confrontation on Brain Function and Cocaine Self-Administration. Neuropsychopharmacology :
Brust, Tarsis F; Morgenweck, Jenny; Kim, Susy A et al. (2016) Biased agonists of the kappa opioid receptor suppress pain and itch without causing sedation or dysphoria. Sci Signal 9:ra117

Showing the most recent 10 out of 291 publications