The Computational Genomics Core in this Center will perform bioinformatic, statistical and pathway analyses of genome-wide expression assays of multiple animal models and human patients, and develop comprehensive knowledgebase of the genomic, proteomic, and metabolic response to burn injury for subsequent disease modeling and target prediction. The overall objective of Core is to use state-of-the-art mathematical and computational approaches needed to better understand the complex systems biology presented by injury and critical illness, as outlined in the Major Functions ofthe Core: 1. Investigate via computational analyses the genomic mechanism ofthe adaptive and maladaptive physiological responses to thermo injury in studies of the Research Projects, including 1 a. Develop computational tools for using new exon-junction arrays in studying animal models (mouse and Rhesus monkeys), lb. Analyze the genomic effect of activation and/or inhibition of genes important to insulin resistance and mitochondrial dysfunction in animal models, and 1c. Compare cross species the genomic changes between animal models and burn patients, and between LPS and burns. 2. Integrate the genomic, protein activity and metabolic data generated by the Program for rational target identification of gene candidates for intervention, including 2a. Develop a knowledgebase of molecular derangements in skeletal muscle following thermal injury by integrating findings from the Research Projects with molecular information systematically harvested from the literature as well as comprehensive human transcriptome data of burn patients, and 2b. Conduct computational analysis to identify key gene regulators for follow up intervention studies candidate serine-threonine kinases for follow-up inhibition. 3. Establish a web-based portal ofthe data and knowledgebase as central community resource.

Public Health Relevance

The Computational Genomics Core will develop and apply mathematical and computational methods to analyze, integrate, and share the large amount of research data and findings from the Center as well as the research community in order to understand the molecular mechanism of burn injury and subsequently help identify new targets for intervention. The ultimate goal is to search for better treatments of thermo injury.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Specialized Center (P50)
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Special Emphasis Panel (ZGM1-SRC-5 (TB))
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Massachusetts General Hospital
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Shank, Erik S; Martyn, Jeevendra A; Donelan, Mathias B et al. (2016) Ultrasound-Guided Regional Anesthesia for Pediatric Burn Reconstructive Surgery: A Prospective Study. J Burn Care Res 37:e213-7
Ueki, Ryusuke; Liu, Li; Kashiwagi, Shizuka et al. (2016) Role of Elevated Fibrinogen in Burn-Induced Mitochondrial Dysfunction: Protective Effects of Glycyrrhizin. Shock 46:382-9
Copps, Kyle D; Hançer, Nancy J; Qiu, Wei et al. (2016) Serine 302 Phosphorylation of Mouse Insulin Receptor Substrate 1 (IRS1) Is Dispensable for Normal Insulin Signaling and Feedback Regulation by Hepatic S6 Kinase. J Biol Chem 291:8602-17
Frydman, Galit H; Bendapudi, Pavan K; Marini, Robert P et al. (2016) Coagulation Biomarkers in Healthy Chinese-Origin Rhesus Macaques (Macaca mulatta). J Am Assoc Lab Anim Sci 55:252-9
Agarwal, Shailesh; Loder, Shawn; Brownley, Cameron et al. (2016) Inhibition of Hif1α prevents both trauma-induced and genetic heterotopic ossification. Proc Natl Acad Sci U S A 113:E338-47
Tompkins, Ronald G (2015) Survival from burns in the new millennium: 70 years' experience from a single institution. Ann Surg 261:263-8
Nakazawa, Harumasa; Yamada, Marina; Tanaka, Tomokazu et al. (2015) Role of protein farnesylation in burn-induced metabolic derangements and insulin resistance in mouse skeletal muscle. PLoS One 10:e0116633
Bittner, Edward A; Shank, Erik; Woodson, Lee et al. (2015) Acute and perioperative care of the burn-injured patient. Anesthesiology 122:448-64
Kashiwagi, Aki; Hosokawa, Sachiko; Maeyama, Yoshihiro et al. (2015) Anesthesia with Disuse Leads to Autophagy Up-regulation in the Skeletal Muscle. Anesthesiology 122:1075-83
White, Morris F (2015) Longevity: Mapping the path to a longer life. Nature 524:170-1

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