The Animal Research Core maintains existing and establishes new protocols for producing burn injury and investigating metabolic patterns and the cellular and molecular changes that occur in murine tissues and blood following burn injury that recapitulate development ofthe hypermetabolic response to injury that occur in human patients with burn injury. The interpretation of results from murine models can help us redesign the models to more closely mimic the situation in human burn patients. Such studies are essential for the development of effective therapeutic strategies to prevent the occurrence of potentially fatal complications in seriously burned patients. This facility ensures that all investigators in the center produce burn injuries with uniform, reproducible results. In addition, we assist investigators in producing trans-genic animals. To facilitate interpretation and comparison of results from the different projects in the center, we strive to have all murine studies performed with animals that have a common genetic background. Preparation for and performance of the complex animal models used in the Burn Trauma Center can be problematic for young investigators and research fellows without substantial previous experience with the techniques involved. In this context the Animal Research Core is extremely helpful in the pre- and post-operative care of study animals and assumes the administrative responsibilities for animal care. This has provided consistency and reproducibility within and between studies and has insured that animal welfare is maintained. Specific services to the Burn Trauma Center that are provided by the Animal Research Core include: 1. maintenance of a central facility and technical staff that is equipped to set-up and maintain uniform, complex animal models of burn injury,. 2, assumption ofthe responsibility that all animal studies funded by the Center are current and up-to-date and that all procedures follow strict humane guidelines, 3. maintenance of a library of books and protocols related to the performance of animal procedures that is freely available to center investigators

Public Health Relevance

The Animal Research Core maintains existing and establishes new protocols for producing burn injury and investigating metabolic patterns and the cellular and molecular changes that occur in murine tissues and blood following burn injury that recapitulate development of the hypermetabolic response to injury that occur in human patients with burn injury.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Specialized Center (P50)
Project #
2P50GM021700-32A1
Application #
8414946
Study Section
Special Emphasis Panel (ZGM1-SRC-5 (TB))
Project Start
Project End
Budget Start
2013-06-01
Budget End
2014-05-31
Support Year
32
Fiscal Year
2013
Total Cost
$230,443
Indirect Cost
$64,238
Name
Massachusetts General Hospital
Department
Type
DUNS #
073130411
City
Boston
State
MA
Country
United States
Zip Code
02199
Nakazawa, Harumasa; Chang, Kyungho; Shinozaki, Shohei et al. (2017) iNOS as a Driver of Inflammation and Apoptosis in Mouse Skeletal Muscle after Burn Injury: Possible Involvement of Sirt1 S-Nitrosylation-Mediated Acetylation of p65 NF-?B and p53. PLoS One 12:e0170391
Khan, Mohammed A S; Khan, Mohammed F; Kashiwagi, Shizuka et al. (2017) An ALPHA7 Nicotinic Acetylcholine Receptor Agonist (GTS-21) Promotes C2C12 Myonuclear Accretion in Association with Release of Interleukin-6 (IL-6) and Improves Survival in Burned Mice. Shock 48:227-235
Frydman, Galit H; Marini, Robert P; Bakthavatchalu, Vasudevan et al. (2017) Local and Systemic Changes Associated with Long-term, Percutaneous, Static Implantation of Titanium Alloys in Rhesus Macaques (Macaca mulatta). Comp Med 67:165-175
Li, Peng; Tompkins, Ronald G; Xiao, Wenzhong et al. (2017) KERIS: kaleidoscope of gene responses to inflammation between species. Nucleic Acids Res 45:D908-D914
Kashiwagi, Shizuka; Khan, Mohammed A S; Yasuhara, Shingo et al. (2017) Prevention of Burn-Induced Inflammatory Responses and Muscle Wasting by GTS-21, a Specific Agonist for ?7 Nicotinic Acetylcholine Receptors. Shock 47:61-69
Frydman, Galit H; Bendapudi, Pavan K; Marini, Robert P et al. (2016) Coagulation Biomarkers in Healthy Chinese-Origin Rhesus Macaques (Macaca mulatta). J Am Assoc Lab Anim Sci 55:252-9
Agarwal, Shailesh; Loder, Shawn; Brownley, Cameron et al. (2016) Inhibition of Hif1? prevents both trauma-induced and genetic heterotopic ossification. Proc Natl Acad Sci U S A 113:E338-47
Ueki, Ryusuke; Liu, Li; Kashiwagi, Shizuka et al. (2016) Role of Elevated Fibrinogen in Burn-Induced Mitochondrial Dysfunction: Protective Effects of Glycyrrhizin. Shock 46:382-9
Copps, Kyle D; Han├žer, Nancy J; Qiu, Wei et al. (2016) Serine 302 Phosphorylation of Mouse Insulin Receptor Substrate 1 (IRS1) Is Dispensable for Normal Insulin Signaling and Feedback Regulation by Hepatic S6 Kinase. J Biol Chem 291:8602-17
Shank, Erik S; Martyn, Jeevendra A; Donelan, Mathias B et al. (2016) Ultrasound-Guided Regional Anesthesia for Pediatric Burn Reconstructive Surgery: A Prospective Study. J Burn Care Res 37:e213-7

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