Two converging technologies?optical Imaging and fluorescent probes for cellular function?are revolutionizing the study of biological structure and the dynamics of chemical and genetic networks in living cells. The emphasis of the imaging facility is two-fold: imaging chemical and structural dynamics in intact cells and tissue, and resolving cell architecture at high spatial resolution. In live cells and tissue imaging, a key technology is two photon laser scanning microscopy (TPLSM). It minimizes phototoxic effects and offers the possibility to image deep into tissues. A set of instruments using this powerful technology Is available in the Imaging Facility. Until recently, ultra-structural studies could only be performed using Electron Microscopy (EM). The last few years have seen extraordinary developments of imaging techniques that are able to break the diffraction barrier by more than an order of magnitude. The advantages of optical microscopy over EM are numerous. A few examples are the possibility to label several targets with different fluorescent markers, and the compatibility with live imaging. Our facility now offers such an instrument and an increasing number of teams are interested in using it.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Specialized Center (P50)
Project #
5P50GM071508-10
Application #
8534154
Study Section
Special Emphasis Panel (ZGM1-CBCB-4)
Project Start
Project End
Budget Start
2013-09-01
Budget End
2014-08-31
Support Year
10
Fiscal Year
2013
Total Cost
$133,979
Indirect Cost
$37,268
Name
Princeton University
Department
Type
DUNS #
002484665
City
Princeton
State
NJ
Country
United States
Zip Code
08544
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