Core Capabilities &Approach: Complexes will be crystallized by vapor diffusion, microbatch, and microdialysis methods. After identification of initial crystallization conditions, optimization will be carried out to obtain high-quality crystals for X-ray diffraction. All crystals will be screened for diffraction quality at the home source;should diffraction be obtained that is not of sufficiently high resolution and/or quality, changes at the protein level will be carried out in the Protein Core. Such an iterative cycle wdll be continued until sufficiently high-quality diffracting crystals become available. Other possible avenues for obtaining better diffracting crystals include i) Identifying precipitant and protein concentrations that minimize showering of small crystals or excessive nucleation;2) Identifying additives that improve resolution and expanding on these classes of chemicals;and 3) using annealing procedures to lower mosaicity and increase resolution. Crystallographic data will be mainly collected at the SERCAT facility sector 22-BM beam line of the Advance Photon Source at Argonne National Laboratory, Chicago, IL, permitting collection of high-resolution diffraction data on native protein complexes and multiple wavelength anomalous diffraction using Se-Met derivative crystals. The selenium atom sites and MAD phases will be automatically determined using the AutoSol module and initial model building with additional iterative density improvement will be done using the Autobuild module in the program Phenix(254-256)^ Xhe initial MAD structure will be used as a Molecular Replacement (MR) model and structures will be further refined through cycles of rebuilding and refinement using the program Coot(257, 258) and RefMac(259) a refinement program within the CCP4 package if necessary, we will carry out heavy atom derivatives to solve the crystallographic phase problem. iii. Proiect Component: In addition to screening activities, the Core wdll determine the structures of complexes containing DCAFi, DDBi, and Vpr/Vpx with associated substrates (for example, SAMHDi), other E3 ligase complexes, capsid related complexes, and Trimsa SPRY domain.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Specialized Center (P50)
Project #
2P50GM082251-06
Application #
8528177
Study Section
Special Emphasis Panel (ZRG1-AARR-K (50))
Project Start
Project End
Budget Start
2012-09-30
Budget End
2013-07-31
Support Year
6
Fiscal Year
2012
Total Cost
$456,957
Indirect Cost
$110,718
Name
University of Pittsburgh
Department
Type
DUNS #
004514360
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213
Quinn, Caitlin M; Polenova, Tatyana (2017) Structural biology of supramolecular assemblies by magic-angle spinning NMR spectroscopy. Q Rev Biophys 50:e1
Akiyama, Hisashi; Ramirez, Nora-Guadalupe Pina; Gibson, Gregory et al. (2017) Interferon-Inducible CD169/Siglec1 Attenuates Anti-HIV-1 Effects of Alpha Interferon. J Virol 91:
Yamashita, Masahiro; Engelman, Alan N (2017) Capsid-Dependent Host Factors in HIV-1 Infection. Trends Microbiol 25:741-755
Wang, Weifeng; Zhou, Jing; Halambage, Upul D et al. (2017) Inhibition of HIV-1 Maturation via Small-Molecule Targeting of the Amino-Terminal Domain in the Viral Capsid Protein. J Virol 91:
Vergara, Sandra; Lukes, Dylan A; Martynowycz, Michael W et al. (2017) MicroED Structure of Au146(p-MBA)57 at Subatomic Resolution Reveals a Twinned FCC Cluster. J Phys Chem Lett 8:5523-5530
Perilla, Juan R; Zhao, Gongpu; Lu, Manman et al. (2017) CryoEM Structure Refinement by Integrating NMR Chemical Shifts with Molecular Dynamics Simulations. J Phys Chem B 121:3853-3863
Wang, Mingzhang; Quinn, Caitlin M; Perilla, Juan R et al. (2017) Quenching protein dynamics interferes with HIV capsid maturation. Nat Commun 8:1779
Ballandras-Colas, Allison; Maskell, Daniel P; Serrao, Erik et al. (2017) A supramolecular assembly mediates lentiviral DNA integration. Science 355:93-95
Zhou, Xiaohong; DeLucia, Maria; Hao, Caili et al. (2017) HIV-1 Vpr protein directly loads helicase-like transcription factor (HLTF) onto the CRL4-DCAF1 E3 ubiquitin ligase. J Biol Chem 292:21117-21127
Alvarez, Frances J D; He, Shaoda; Perilla, Juan R et al. (2017) CryoEM structure of MxB reveals a novel oligomerization interface critical for HIV restriction. Sci Adv 3:e1701264

Showing the most recent 10 out of 128 publications