Imaging Core, directed by Zandrea Ambrose and Simon Watkins with collaborative effort from Peijun Zhang. i. Service mission: The Imaging Core will visualize the intracellular dynamics of HIV-i components during the processes of HIV-l CA core uncoating, RTxn, and PIC nuclear entry, by live-cell and high-resolution fixed cell microscopy, using existing and novel technologies. The Core will use labeled virions and host cell proteins of PCHPI-selected interest for conducting imaging studies and will develop novel techniques to track postentry events, visualizing viral protein and nucleic acids that interact with host proteins in vivo, using a complete array of super-resolution, optical sectioning, and wide field approaches in close collaboration with the technology development program.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Specialized Center (P50)
Project #
2P50GM082251-06
Application #
8528180
Study Section
Special Emphasis Panel (ZRG1-AARR-K (50))
Project Start
Project End
Budget Start
2012-09-30
Budget End
2013-07-31
Support Year
6
Fiscal Year
2012
Total Cost
$456,957
Indirect Cost
$110,718
Name
University of Pittsburgh
Department
Type
DUNS #
004514360
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213
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