The Tissue EM Core performs high-quality preservation of HIV-infected tissues from both humanized mouse model systems and actual human tissues from biopsies and explants. These samples are plastic-embedded, serially sectioned and imaged for tomographic analysis in either of two electron microscopes. Tomographic reconstructions provide three-dimensional, high-resolution studies of wild-type HIV within actual infected tissues and organs and may shed light on the fine structures involved in HIV budding and evasion from HAART therapy. The Core has most equipment necessary for processing, imaging and analyzing tissue samples. The core has effectively unlimited access to two tomography-ready electron microscopes: A FEI Tecnai T12 (120KeV) microscope for sections <200nm in the Caltech Broad Center EM Suite (see EM Tomography Core, Grant Jensen Director) and a newly retrofitted Tecnai TF30-FEG (300KeV) microscope for 300-400nm sections located in the Caltech Material Sciences Department.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Specialized Center (P50)
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Special Emphasis Panel (ZRG1-AARR-K)
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University of Utah
Salt Lake City
United States
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Sanchez, Jacint G; Okreglicka, Katarzyna; Chandrasekaran, Viswanathan et al. (2014) The tripartite motif coiled-coil is an elongated antiparallel hairpin dimer. Proc Natl Acad Sci U S A 111:2494-9
Ren, Jihui; Pei-Chen Lin, Coney; Pathak, Manish C et al. (2014) A phosphatidylinositol transfer protein integrates phosphoinositide signaling with lipid droplet metabolism to regulate a developmental program of nutrient stress-induced membrane biogenesis. Mol Biol Cell 25:712-27
Purdy, Michael D; Bennett, Brad C; McIntire, William E et al. (2014) Function and dynamics of macromolecular complexes explored by integrative structural and computational biology. Curr Opin Struct Biol 27:138-48
Kovalskyy, Dmytro B; Ivanov, Dmitri N (2014) Recognition of the HIV capsid by the TRIM5? restriction factor is mediated by a subset of pre-existing conformations of the TRIM5? SPRY domain. Biochemistry 53:1466-76
Monroe, Nicole; Han, Han; Gonciarz, Malgorzata D et al. (2014) The oligomeric state of the active Vps4 AAA ATPase. J Mol Biol 426:510-25
Ladinsky, Mark S; Kieffer, Collin; Olson, Gregory et al. (2014) Electron tomography of HIV-1 infection in gut-associated lymphoid tissue. PLoS Pathog 10:e1003899
Narayan, Kedar; Danielson, Cindy M; Lagarec, Ken et al. (2014) Multi-resolution correlative focused ion beam scanning electron microscopy: applications to cell biology. J Struct Biol 185:278-84
Hulme, Amy E; Hope, Thomas J (2014) The cyclosporin A washout assay to detect HIV-1 uncoating in infected cells. Methods Mol Biol 1087:37-46
Lamorte, Louie; Titolo, Steve; Lemke, Christopher T et al. (2013) Discovery of novel small-molecule HIV-1 replication inhibitors that stabilize capsid complexes. Antimicrob Agents Chemother 57:4622-31
Votteler, Jorg; Sundquist, Wesley I (2013) Virus budding and the ESCRT pathway. Cell Host Microbe 14:232-41

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