EM Tomography Core (Jensen. Director: Tivol &Ding. Managers) Overview The EM Tomography Core will be housed within the Jensen lab at the California Institute of Technology in Pasadena, California. Two electron cryomicroscopes are present there: a 300kV, FEG, liquid heliumcooled, energy filtered, dual-axis tilting, fully automated "G2 Polara", and a 120 kV, LaB6, liquid nitrogencooled "Tecnai T12." The EM Tomography Core produces 3D reconstrucfions of unique samples such as individual virions or cells to macromolecular (~2-6 nm) resolution. Two cryoelectron microscopes are used, a 300-kV Polara and a 120- kV T12, both from the FEI Company. Samples are either plunge-frozen intact in liquid ethane or high-pressure frozen and then cryosecfioned. Tilt-series are acquired automafically and then combined computafionally to produce the 3-D reconstrucfions.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Specialized Center (P50)
Project #
Application #
Study Section
Special Emphasis Panel (ZRG1-AARR-K)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Utah
Salt Lake City
United States
Zip Code
Sanchez, Jacint G; Okreglicka, Katarzyna; Chandrasekaran, Viswanathan et al. (2014) The tripartite motif coiled-coil is an elongated antiparallel hairpin dimer. Proc Natl Acad Sci U S A 111:2494-9
Ren, Jihui; Pei-Chen Lin, Coney; Pathak, Manish C et al. (2014) A phosphatidylinositol transfer protein integrates phosphoinositide signaling with lipid droplet metabolism to regulate a developmental program of nutrient stress-induced membrane biogenesis. Mol Biol Cell 25:712-27
Purdy, Michael D; Bennett, Brad C; McIntire, William E et al. (2014) Function and dynamics of macromolecular complexes explored by integrative structural and computational biology. Curr Opin Struct Biol 27:138-48
Kovalskyy, Dmytro B; Ivanov, Dmitri N (2014) Recognition of the HIV capsid by the TRIM5? restriction factor is mediated by a subset of pre-existing conformations of the TRIM5? SPRY domain. Biochemistry 53:1466-76
Monroe, Nicole; Han, Han; Gonciarz, Malgorzata D et al. (2014) The oligomeric state of the active Vps4 AAA ATPase. J Mol Biol 426:510-25
Ladinsky, Mark S; Kieffer, Collin; Olson, Gregory et al. (2014) Electron tomography of HIV-1 infection in gut-associated lymphoid tissue. PLoS Pathog 10:e1003899
Narayan, Kedar; Danielson, Cindy M; Lagarec, Ken et al. (2014) Multi-resolution correlative focused ion beam scanning electron microscopy: applications to cell biology. J Struct Biol 185:278-84
Hulme, Amy E; Hope, Thomas J (2014) The cyclosporin A washout assay to detect HIV-1 uncoating in infected cells. Methods Mol Biol 1087:37-46
Lamorte, Louie; Titolo, Steve; Lemke, Christopher T et al. (2013) Discovery of novel small-molecule HIV-1 replication inhibitors that stabilize capsid complexes. Antimicrob Agents Chemother 57:4622-31
Votteler, Jorg; Sundquist, Wesley I (2013) Virus budding and the ESCRT pathway. Cell Host Microbe 14:232-41

Showing the most recent 10 out of 66 publications