EM Tomography Core (Jensen. Director: Tivol &Ding. Managers) Overview The EM Tomography Core will be housed within the Jensen lab at the California Institute of Technology in Pasadena, California. Two electron cryomicroscopes are present there: a 300kV, FEG, liquid heliumcooled, energy filtered, dual-axis tilting, fully automated "G2 Polara", and a 120 kV, LaB6, liquid nitrogencooled "Tecnai T12." The EM Tomography Core produces 3D reconstrucfions of unique samples such as individual virions or cells to macromolecular (~2-6 nm) resolution. Two cryoelectron microscopes are used, a 300-kV Polara and a 120- kV T12, both from the FEI Company. Samples are either plunge-frozen intact in liquid ethane or high-pressure frozen and then cryosecfioned. Tilt-series are acquired automafically and then combined computafionally to produce the 3-D reconstrucfions.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Specialized Center (P50)
Project #
5P50GM082545-08
Application #
8728906
Study Section
Special Emphasis Panel (ZRG1-AARR-K)
Project Start
Project End
Budget Start
2014-08-01
Budget End
2015-07-31
Support Year
8
Fiscal Year
2014
Total Cost
$378,754
Indirect Cost
Name
University of Utah
Department
Type
DUNS #
009095365
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112
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