The Tissue EM Core performs high-quality preservation of HIV-infected tissues from both humanized mouse model systems and actual human tissues from biopsies and explants. These samples are plastic-embedded, serially sectioned and imaged for tomographic analysis in either of two electron microscopes. Tomographic reconstructions provide three-dimensional, high-resolution studies of wild-type HIV within actual infected tissues and organs and may shed light on the fine structures involved in HIV budding and evasion from HAART therapy. The Core has most equipment necessary for processing, imaging and analyzing tissue samples. The core has effectively unlimited access to two tomography-ready electron microscopes: A FEI Tecnai T12 (120KeV) microscope for sections <200nm in the Caltech Broad Center EM Suite (see EM Tomography Core, Grant Jensen Director) and a newly retrofitted Tecnai TF30-FEG (300KeV) microscope for 300-400nm sections located in the Caltech Material Sciences Department.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Specialized Center (P50)
Project #
5P50GM082545-08
Application #
8728913
Study Section
Special Emphasis Panel (ZRG1-AARR-K)
Project Start
Project End
Budget Start
2014-08-01
Budget End
2015-07-31
Support Year
8
Fiscal Year
2014
Total Cost
$297,523
Indirect Cost
Name
University of Utah
Department
Type
DUNS #
009095365
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112
Wang, Haoqing; Cohen, Alexander A; Galimidi, Rachel P et al. (2016) Cryo-EM structure of a CD4-bound open HIV-1 envelope trimer reveals structural rearrangements of the gp120 V1V2 loop. Proc Natl Acad Sci U S A 113:E7151-E7158
Monroe, Nicole; Hill, Christopher P (2016) Meiotic Clade AAA ATPases: Protein Polymer Disassembly Machines. J Mol Biol 428:1897-911
Imam, Sabrina; Talley, Sarah; Nelson, Rachel S et al. (2016) TRIM5α Degradation via Autophagy Is Not Required for Retroviral Restriction. J Virol 90:3400-10
Grime, John M A; Dama, James F; Ganser-Pornillos, Barbie K et al. (2016) Coarse-grained simulation reveals key features of HIV-1 capsid self-assembly. Nat Commun 7:11568
Chen, Jianbo; Rahman, Sheikh Abdul; Nikolaitchik, Olga A et al. (2016) HIV-1 RNA genome dimerizes on the plasma membrane in the presence of Gag protein. Proc Natl Acad Sci U S A 113:E201-8
Chuong, Edward B; Elde, Nels C; Feschotte, Cédric (2016) Regulatory evolution of innate immunity through co-option of endogenous retroviruses. Science 351:1083-7
Ma, Hanhui; Tu, Li-Chun; Naseri, Ardalan et al. (2016) Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nat Biotechnol 34:528-30
Iwasa, Janet H (2016) The Scientist as Illustrator. Trends Immunol 37:247-50
Li, Yen-Li; Chandrasekaran, Viswanathan; Carter, Stephen D et al. (2016) Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids. Elife 5:
Coey, Aaron; Larsen, Kevin; Puglisi, Joseph D et al. (2016) Heterogeneous structures formed by conserved RNA sequences within the HIV reverse transcription initiation site. RNA 22:1689-1698

Showing the most recent 10 out of 130 publications