Gnotobiology Core: The Gnotobiology Core will rear germ-free zebrafish and stickleback in designated space within the UO Zebrafish Facility. This space will house sterile isolators in proximity to a fume hood for ventilation of Clidox gas used for sterilization. There is also a biosafety cabinet for aseptic manipulation of zebrafish. The Gnotobiology Core staff will have access to a new autoclave featuring custom programs optimized for sterilization of liquid food.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Specialized Center (P50)
Project #
5P50GM098911-03
Application #
8727612
Study Section
Special Emphasis Panel (ZGM1)
Project Start
Project End
Budget Start
2014-09-01
Budget End
2015-08-31
Support Year
3
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Oregon
Department
Type
DUNS #
City
Eugene
State
OR
Country
United States
Zip Code
97403
Parthasarathy, Raghuveer (2017) Monitoring microbial communities using light sheet fluorescence microscopy. Curr Opin Microbiol 43:31-37
Burns, Adam R; Guillemin, Karen (2017) The scales of the zebrafish: host-microbiota interactions from proteins to populations. Curr Opin Microbiol 38:137-141
Melancon, E; Gomez De La Torre Canny, S; Sichel, S et al. (2017) Best practices for germ-free derivation and gnotobiotic zebrafish husbandry. Methods Cell Biol 138:61-100
Jones, Tiffani Alvey; Hernandez, Diane Z; Wong, Zoƫ C et al. (2017) The bacterial virulence factor CagA induces microbial dysbiosis that contributes to excessive epithelial cell proliferation in the Drosophila gut. PLoS Pathog 13:e1006631
Stagaman, Keaton; Burns, Adam R; Guillemin, Karen et al. (2017) The role of adaptive immunity as an ecological filter on the gut microbiota in zebrafish. ISME J 11:1630-1639
Small, Clayton M; Milligan-Myhre, Kathryn; Bassham, Susan et al. (2017) Host Genotype and Microbiota Contribute Asymmetrically to Transcriptional Variation in the Threespine Stickleback Gut. Genome Biol Evol 9:504-520
Taormina, Michael J; Hay, Edouard A; Parthasarathy, Raghuveer (2017) Passive and Active Microrheology of the Intestinal Fluid of the Larval Zebrafish. Biophys J 113:957-965
Burns, A R; Watral, V; Sichel, S et al. (2017) Transmission of a common intestinal neoplasm in zebrafish by cohabitation. J Fish Dis :
Stephens, W Zac; Burns, Adam R; Stagaman, Keaton et al. (2016) The composition of the zebrafish intestinal microbial community varies across development. ISME J 10:644-54
Burns, Adam R; Stephens, W Zac; Stagaman, Keaton et al. (2016) Contribution of neutral processes to the assembly of gut microbial communities in the zebrafish over host development. ISME J 10:655-64

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