Abstract

The objectives of this Core are: to maintain a resource laboratory for measuring eicosanoid and cytokine concentrations in biologic fluids; to share costly resources required for studying the biochemistry of arachidonic acid metabolites and cytokines; and to provide consultation to SCOR investigators regarding suitable methods and experimental designs for the collection and measurement of these mediators. Using equilibrium radioimmunoassay techniques, the laboratory will measure prostaglandins (PGE2, PGF2delta, PGD2, and the stable hydrolysis products of PGI2 (6-Keto- PGF1delta) and thromboxane A2 (thromboxane B2, TxB2), 12 and 5 hydroxyeicosatetraenoic acid (12 and 5 HETE), and the leukotrienes C4 (LTC4) and D4 (LTD4) in lung washings and cell culture fluids. Employing solid phase immunoassays in concert with biological assays, cytokines present in biological fluids will be quantitated by uniform methodology. A central core laboratory will, therefore, consolidate costs, achieve efficient processing of samples, and provide quality control and direct consultation for investigators participating in SCOR research activities.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Specialized Center (P50)
Project #
2P50HL037117-06
Application #
3844547
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
6
Fiscal Year
1992
Total Cost
Indirect Cost

  Institution

Name
Case Western Reserve University
Department
Type
DUNS #
077758407
City
Cleveland
State
OH
Country
United States
Zip Code
44106

  Publications

Kargacin, Gary J; Hunt, Donald; Emmett, Teresa et al. (2006) Localization of telokin at the intercalated discs of cardiac myocytes. Arch Biochem Biophys 456:151-60
McLane, M L; Nelson, J A; Lenner, K A et al. (2000) Integrated response of the upper and lower respiratory tract of asthmatic subjects to frigid air. J Appl Physiol 88:1043-50
Korosec, M; Novak, R D; Myers, E et al. (1999) Salmeterol does not compromise the bronchodilator response to albuterol during acute episodes of asthma. Am J Med 107:209-13
Bonfield, T L; Konstan, M W; Berger, M (1999) Altered respiratory epithelial cell cytokine production in cystic fibrosis. J Allergy Clin Immunol 104:72-8
McFadden Jr, E R (1998) Inhaled glucocorticoids and acute asthma: therapeutic breakthrough or nonspecific effect? Am J Respir Crit Care Med 157:677-8
Scott-Woo, G C; Walsh, M P; Ikebe, M et al. (1998) Identification and localization of caldesmon in cardiac muscle. Biochem J 334 ( Pt 1):161-70
Blanchard, T G; Czinn, S J (1998) Review article: Immunological determinants that may affect the Helicobacter pylori cancer risk. Aliment Pharmacol Ther 12 Suppl 1:83-90
McFadden Jr, E R; Strauss, L; Hejal, R et al. (1998) Comparison of two dosage regimens of albuterol in acute asthma. Am J Med 105:12-7
Sompradeekul, S; Hejal, R; McLane, M et al. (1998) Lack of interaction of hyperpnoea with methacholine and histamine in asthma. Clin Sci (Lond) 95:611-9
Czinn, S J; Nedrud, J G (1997) Immunopathology of Helicobacter pylori infection and disease. Springer Semin Immunopathol 18:495-513

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