Recent advances in imaging now provide us with an outstanding opportunity to improve our knowledge about the relationship between cellular function and physiology of the airspaces in patients with asthma. The primary mission of the Imaging Core is to correlate changes in airway structure, physiology, and pathology with function of the recruited cell. The imaging data gained from the human and animal studies will be correlated with physiologic and cellular data obtained in Projects I-V.
Our specific aims are to; 1) evaluate the influence of eosinophils on airway physiology using hyperpolarized helium-3 (3He) MR imaging of lung ventilation in human and animal models of asthma, 2) determine the regional distribution of metabolically active eosinophils in human and animal models of asthma using 18FDG PET, and 3) co-register the metabolic and physiologic data from PET and MRI using anatomic information from high-resolution CT scanning of the lungs. Since many of the proposed techniques are new, CORE C will be responsible for developing and performing the proposed imaging methods.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Specialized Center (P50)
Project #
2P50HL056396-06
Application #
6573131
Study Section
Special Emphasis Panel (ZHL1-CSR-H (M1))
Project Start
2002-01-05
Project End
2006-11-30
Budget Start
2002-01-05
Budget End
2002-11-30
Support Year
6
Fiscal Year
2002
Total Cost
$243,652
Indirect Cost
Name
University of Wisconsin Madison
Department
Type
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
Kelly, Elizabeth A; Esnault, Stephane; Johnson, Sean H et al. (2016) Human eosinophil activin A synthesis and mRNA stabilization are induced by the combination of IL-3 plus TNF. Immunol Cell Biol 94:701-8
Lee, Yong Gyu; Jeong, Jong Jin; Nyenhuis, Sharmilee et al. (2015) Recruited alveolar macrophages, in response to airway epithelial-derived monocyte chemoattractant protein 1/CCl2, regulate airway inflammation and remodeling in allergic asthma. Am J Respir Cell Mol Biol 52:772-84
Park, Gye Young; Lee, Yong Gyu; Berdyshev, Evgeny et al. (2013) Autotaxin production of lysophosphatidic acid mediates allergic asthmatic inflammation. Am J Respir Crit Care Med 188:928-40
Sorkness, Ronald L; Szakaly, Renee J; Rosenthal, Louis A et al. (2013) Viral bronchiolitis in young rats causes small airway lesions that correlate with reduced lung function. Am J Respir Cell Mol Biol 49:808-13
Denlinger, Loren C; Kelly, Elizabeth A B; Dodge, Ann M et al. (2013) Safety of and cellular response to segmental bronchoprovocation in allergic asthma. PLoS One 8:e51963
Gavala, M L; Kelly, E A B; Esnault, S et al. (2013) Segmental allergen challenge enhances chitinase activity and levels of CCL18 in mild atopic asthma. Clin Exp Allergy 43:187-97
Oh, Jiyoung; Malter, James S (2013) Pin1-FADD interactions regulate Fas-mediated apoptosis in activated eosinophils. J Immunol 190:4937-45
Ochkur, Sergei I; Kim, John Dongil; Protheroe, Cheryl A et al. (2012) A sensitive high throughput ELISA for human eosinophil peroxidase: a specific assay to quantify eosinophil degranulation from patient-derived sources. J Immunol Methods 384:10-20
Curran, Colleen S; Bertics, Paul J (2012) Lactoferrin regulates an axis involving CD11b and CD49d integrins and the chemokines MIP-1? and MCP-1 in GM-CSF-treated human primary eosinophils. J Interferon Cytokine Res 32:450-61
Kelly, Elizabeth A B; Liu, Lin Ying; Esnault, Stephane et al. (2012) Potent synergistic effect of IL-3 and TNF on matrix metalloproteinase 9 generation by human eosinophils. Cytokine 58:199-206

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