Abstract

The goal of CORE B is to provide high purity eosinophil (EOS) preparations and to perform a variety of assays to assess cell function when placed in the different environments of Projects II, III, IV and V. It will be the aim of this facility to: 1. Provide pure peripheral EOS from normal subjects and patients with allergic rhinitis or allergic asthma, to provide pure bronchoalveolar lavage (BAL) EOS following segmental bronchoprovocation with allergen (SBP-AG) or inhaled corticosteroid (ICS) withdrawal; 2. Provide pure rat peritoneal EOS from study animals in Project II; 3. Perform functional assessment of human and rat EOS biology; 4. Adapt current protocols to the requirements of each project; and 5. Teach functional protocols to personnel of Projects II-V or to perform techniques necessary for specific studies.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Specialized Center (P50)
Project #
5P50HL056396-09
Application #
7007651
Study Section
Project Start
Project End
Budget Start
2004-12-01
Budget End
2005-11-30
Support Year
9
Fiscal Year
2005
Total Cost
$267,277
Indirect Cost

  Institution

Name
University of Wisconsin Madison
Department
Type
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715

  Publications

Kelly, Elizabeth A; Esnault, Stephane; Johnson, Sean H et al. (2016) Human eosinophil activin A synthesis and mRNA stabilization are induced by the combination of IL-3 plus TNF. Immunol Cell Biol 94:701-8
Lee, Yong Gyu; Jeong, Jong Jin; Nyenhuis, Sharmilee et al. (2015) Recruited alveolar macrophages, in response to airway epithelial-derived monocyte chemoattractant protein 1/CCl2, regulate airway inflammation and remodeling in allergic asthma. Am J Respir Cell Mol Biol 52:772-84
Park, Gye Young; Lee, Yong Gyu; Berdyshev, Evgeny et al. (2013) Autotaxin production of lysophosphatidic acid mediates allergic asthmatic inflammation. Am J Respir Crit Care Med 188:928-40
Sorkness, Ronald L; Szakaly, Renee J; Rosenthal, Louis A et al. (2013) Viral bronchiolitis in young rats causes small airway lesions that correlate with reduced lung function. Am J Respir Cell Mol Biol 49:808-13
Denlinger, Loren C; Kelly, Elizabeth A B; Dodge, Ann M et al. (2013) Safety of and cellular response to segmental bronchoprovocation in allergic asthma. PLoS One 8:e51963
Gavala, M L; Kelly, E A B; Esnault, S et al. (2013) Segmental allergen challenge enhances chitinase activity and levels of CCL18 in mild atopic asthma. Clin Exp Allergy 43:187-97
Oh, Jiyoung; Malter, James S (2013) Pin1-FADD interactions regulate Fas-mediated apoptosis in activated eosinophils. J Immunol 190:4937-45
Ochkur, Sergei I; Kim, John Dongil; Protheroe, Cheryl A et al. (2012) A sensitive high throughput ELISA for human eosinophil peroxidase: a specific assay to quantify eosinophil degranulation from patient-derived sources. J Immunol Methods 384:10-20
Curran, Colleen S; Bertics, Paul J (2012) Lactoferrin regulates an axis involving CD11b and CD49d integrins and the chemokines MIP-1? and MCP-1 in GM-CSF-treated human primary eosinophils. J Interferon Cytokine Res 32:450-61
Kelly, Elizabeth A B; Liu, Lin Ying; Esnault, Stephane et al. (2012) Potent synergistic effect of IL-3 and TNF on matrix metalloproteinase 9 generation by human eosinophils. Cytokine 58:199-206

Showing the most recent 10 out of 112 publications