Abstract

The Gene Therapy Primate Core will be located in the Department of Physiology and Medicine at the Southwest Foundation for Biomedical Research in San Antonio, Texas. This core will be responsible for supplying normal and LDL-R defective rhesus monkeys, administering experimental treatments, maintaining subjects that are shedding virus under appropriate containment (biosafety level 2), sampling animals regularly to determine the duration of viral shedding and drawing blood to measure gene expression and lipoprotein kinetics, necropsing animals to gather materials to measure final endpoints, assessing vascular lesions in control and treated animals, and preparing and shipping samples to Baylor College of Medicine in Houston.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Specialized Center (P50)
Project #
5P50HL059314-03
Application #
6258932
Study Section
Project Start
1999-09-01
Project End
2000-08-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
3
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
Baylor College of Medicine
Department
Type
DUNS #
074615394
City
Houston
State
TX
Country
United States
Zip Code
77030

  Publications

Oka, K; Mullins, C E; Kushwaha, R S et al. (2015) Gene therapy for rhesus monkeys heterozygous for LDL receptor deficiency by balloon catheter hepatic delivery of helper-dependent adenoviral vector. Gene Ther 22:87-95
Palmer, Donna J; Ng, Philip (2011) Rescue, amplification, and large-scale production of helper-dependent adenoviral vectors. Cold Spring Harb Protoc 2011:857-66
Palmer, Donna J; Ng, Philip (2011) Characterization of helper-dependent adenoviral vectors. Cold Spring Harb Protoc 2011:867-70
Stephen, Sam Laurel; Montini, Eugenio; Sivanandam, Vijayshankar Ganesh et al. (2010) Chromosomal integration of adenoviral vector DNA in vivo. J Virol 84:9987-94
Sakai, Keiko; Tiebel, Oliver; Ljungberg, M Cecilia et al. (2009) A neuronal VLDLR variant lacking the third complement-type repeat exhibits high capacity binding of apoE containing lipoproteins. Brain Res 1276:11-21
Brunetti-Pierri, Nicola; Stapleton, Gary E; Law, Mark et al. (2009) Efficient, long-term hepatic gene transfer using clinically relevant HDAd doses by balloon occlusion catheter delivery in nonhuman primates. Mol Ther 17:327-33
Palmer, Donna J; Ng, Philip (2008) Methods for the production of helper-dependent adenoviral vectors. Methods Mol Biol 433:33-53
Palmer, Donna J; Ng, Philip (2008) Methods for the production of first generation adenoviral vectors. Methods Mol Biol 433:55-78
Brunetti-Pierri, Nicola; Stapleton, Gary E; Palmer, Donna J et al. (2007) Pseudo-hydrodynamic delivery of helper-dependent adenoviral vectors into non-human primates for liver-directed gene therapy. Mol Ther 15:732-40
Oka, K; Belalcazar, L M; Dieker, C et al. (2007) Sustained phenotypic correction in a mouse model of hypoalphalipoproteinemia with a helper-dependent adenovirus vector. Gene Ther 14:191-202

Showing the most recent 10 out of 23 publications