The Cell Biology/Morphology Core (Core D) provides a full array of biologic imaging services to aid COPD SCCOR investigators requiring photographic and micrographic documentation (visible, fluorescence, and electron) of basic and clinical research data. In addition to imaging services, the Core makes available its experience with sample handling and detection systems (immunohistochemistry, immunofluorescence, in situ hybridization, scanning and transmission electron microscopy) required to analyze gene and protein expression in experimental samples. The staff of the Core will work with project investigators to develop new applications and analyses for data generated in the COPD SCORR. Specific examples of Core D contributions will include assistance to the Projects in quantification of cell types in the populations of large and small airway epithelium and bronchoalveolar lavage samples that are the starting material for the analyses of gene expression, carrying out immunohistochemical studies on the airway epithelial and alveolar macrophage populations to to assess the expression of selected genes at the morphologic level, analysis of bronchial biopsies to quantify the proportion of cell types to compare to the brushed samples, immunohistochemical analysis of the expression of selected genes, cell cycle analysis, analysis of the morphology of ciliated cells at the light microscopy level and of cilia perse using transmission electron microscopy, determination of cilia beat frequency, and quantitation of the extent of emphysema in the models of lung regeneration and to quantify the proportion of parenchymal cells contributed by circulating stem cells.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Specialized Center (P50)
Project #
5P50HL084936-03
Application #
8114919
Study Section
Special Emphasis Panel (ZHL1)
Project Start
Project End
Budget Start
2009-01-01
Budget End
2009-12-31
Support Year
3
Fiscal Year
2009
Total Cost
Indirect Cost
Name
Weill Medical College of Cornell University
Department
Type
DUNS #
060217502
City
New York
State
NY
Country
United States
Zip Code
10065
Strulovici-Barel, Yael; Staudt, Michelle R; Krause, Anja et al. (2016) Persistence of circulating endothelial microparticles in COPD despite smoking cessation. Thorax 71:1137-1144
Barjaktarevic, Igor Z; Crystal, Ronald G; Kaner, Robert J (2016) The Role of Interleukin-23 in the Early Development of Emphysema in HIV1(+) Smokers. J Immunol Res 2016:3463104
Harvey, Ben-Gary; Strulovici-Barel, Yael; Kaner, Robert J et al. (2015) Risk of COPD with obstruction in active smokers with normal spirometry and reduced diffusion capacity. Eur Respir J 46:1589-1597
Gomi, Kazunori; Arbelaez, Vanessa; Crystal, Ronald G et al. (2015) Activation of NOTCH1 or NOTCH3 signaling skews human airway basal cell differentiation toward a secretory pathway. PLoS One 10:e0116507
Wang, Guoqing; Wang, Rui; Strulovici-Barel, Yael et al. (2015) Persistence of smoking-induced dysregulation of miRNA expression in the small airway epithelium despite smoking cessation. PLoS One 10:e0120824
Walters, Matthew S; De, Bishnu P; Salit, Jacqueline et al. (2014) Smoking accelerates aging of the small airway epithelium. Respir Res 15:94
Hessel, Justina; Heldrich, Jonna; Fuller, Jennifer et al. (2014) Intraflagellar transport gene expression associated with short cilia in smoking and COPD. PLoS One 9:e85453
Shaykhiev, Renat; Crystal, Ronald G (2014) Early events in the pathogenesis of chronic obstructive pulmonary disease. Smoking-induced reprogramming of airway epithelial basal progenitor cells. Ann Am Thorac Soc 11 Suppl 5:S252-8
Brekman, Angelika; Walters, Matthew S; Tilley, Ann E et al. (2014) FOXJ1 prevents cilia growth inhibition by cigarette smoke in human airway epithelium in vitro. Am J Respir Cell Mol Biol 51:688-700
Gao, Chuan; Tignor, Nicole L; Salit, Jacqueline et al. (2014) HEFT: eQTL analysis of many thousands of expressed genes while simultaneously controlling for hidden factors. Bioinformatics 30:369-76

Showing the most recent 10 out of 75 publications