The Vanderbilt Conte Center for Neuroscience Research Enduring Impact of Early-Life Serotonin Signaling involves a set of highly interdependent projects targeting genetic, molecular, biochemical and cellular facets of serotonin (5-HT) neuronal identity, development, signaling, physiology and behavior. The Bioanalytical Core provides a set of physical resources in dedicated space accompanied by dedicated technical staff to support the efforts of Conte Center projects. The Bioanalytical Core, under the directorship of Dr. Ariel Deutch, Professor of Psychiatry and Pharmacology at Vanderbilt, organizes research support on behalf of Conte Investigators in the areas of neurochemical, receptor, and transport assays, protein analyses, and mouse genotyping. Neurochemical analyses include RIA assays to measure signal transduction molecules and neurotransmitter release assays using in vivo microdialysis or in vitro slice suprafusion approaches. Receptor assays include radioligand binding to isolated brain membranes or autoradiography analysis of mouse brain sections. The Core also provides support for the genotyping of mouse lines used in each of the projects. Research space dedicated to core activities in the MRBIII building contains instrumentation and bench space for daily activities related to support activities and includes a microdialysis laboratory room to provide a quiet environment for the measurement of extracellular neurotransmitters in awake, freely moving mice. As with our prior application, Drs. Deutch and Gresch coordinate research support activities with the ongoing pace and priorities of Conte projects and provide training of junior scientists to gain experience in laboratory methodologies that can facilitate their professional development. The detailed methods and resources offered by the Core are shared with Conte investigators and the scientific community through the Conte website and demonstrated through one-on-one interactions with project staff. Finally, the Core provides support for Conte pilot grantees to insure the optimum use of pilot resources and the progression of projects toward more sustained research support.

Public Health Relevance

The Conte Center projects proposed seek an advanced understanding of the molecular and physiological impact of altered 5-HT signaling capacity during development. To achieve this goal, research support activities are needed that extend each project's investigations and which bring skills that either do not exist or which are more efficiently provided through the Bioanalytical Core's contributions.

National Institute of Health (NIH)
National Institute of Mental Health (NIMH)
Specialized Center (P50)
Project #
Application #
Study Section
Special Emphasis Panel (ZMH1-ERB-S)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Vanderbilt University Medical Center
United States
Zip Code
Ulbricht, Randi J; Emeson, Ronald B (2014) One hundred million adenosine-to-inosine RNA editing sites: hearing through the noise. Bioessays 36:730-5
Ciarleglio, Christopher M; Resuehr, Holly E S; Axley, John C et al. (2014) Pet-1 deficiency alters the circadian clock and its temporal organization of behavior. PLoS One 9:e97412
Jackson, Chad R; Capozzi, Megan; Dai, Heng et al. (2014) Circadian perinatal photoperiod has enduring effects on retinal dopamine and visual function. J Neurosci 34:4627-33
Hood, Jennifer L; Morabito, Michael V; Martinez 3rd, Charles R et al. (2014) Reovirus-mediated induction of ADAR1 (p150) minimally alters RNA editing patterns in discrete brain regions. Mol Cell Neurosci 61:97-109
Spoida, Katharina; Masseck, Olivia A; Deneris, Evan S et al. (2014) Gq/5-HT2c receptor signals activate a local GABAergic inhibitory feedback circuit to modulate serotonergic firing and anxiety in mice. Proc Natl Acad Sci U S A 111:6479-84
Ye, R; Carneiro, A M D; Airey, D et al. (2014) Evaluation of heritable determinants of blood and brain serotonin homeostasis using recombinant inbred mice. Genes Brain Behav 13:247-60
Anastasio, Noelle C; Stutz, Sonja J; Fox, Robert G et al. (2014) Functional status of the serotonin 5-HT2C receptor (5-HT2CR) drives interlocked phenotypes that precipitate relapse-like behaviors in cocaine dependence. Neuropsychopharmacology 39:370-82
Deneris, Evan S; Hobert, Oliver (2014) Maintenance of postmitotic neuronal cell identity. Nat Neurosci 17:899-907
Shi, Zhiao; Wang, Jing; Zhang, Bing (2013) NetGestalt: integrating multidimensional omics data over biological networks. Nat Methods 10:597-8
Wu, Hsiao-Huei; Levitt, Pat (2013) Prenatal expression of MET receptor tyrosine kinase in the fetal mouse dorsal raphe nuclei and the visceral motor/sensory brainstem. Dev Neurosci 35:1-16

Showing the most recent 10 out of 11 publications