The Conte Center Molecular Neuroanatomy Core (MNC) will provide high quality neuroanatomical services for both qualitative and quantitative observations of gene and protein expression, cell counts, and basic measures of regional morphometry. The core will support the research efforts of the Vanderbilt Conte Center for Basic Neuroscience by providing a number of key services, including: 1) housing of a reference library collection of wild type brain sections for more rapid optimization of staining protocols for in situ hybridization and immunocytochemistry;2) generation of comparative mutant and wild type series of stained material based on needs of each investigator for localization of specific proteins and transcripts;3) basic histological staining of wild type and mutant mouse brain material for initial analysis of brain structure;4) Microbrightfield-based computer-assisted basic quantitative morphometry and assorted measures that include signal intensity measures, cell counts based on stereological techniques, area fraction measures of regional serotonin innervation. The quantitative morphometric analyses will be done for investigators as pilot projects to determine the extent of more detailed studies needed. The analytical data provided by the core will assist in the design of detailed analyses by Conte investigators. The core will provide assistance for investigator laboratories to learn how to use morphometric analysis tools on exported datasets using local workstations. The MNC has the capacity to section between 300-400 brains annually, and performing immunocytochemistry or in situ hybridization on a subset of sections from an equivalent number of brains Core leadership and personnel have substantial experience utilizing neuroanatomical methods for examining gene and protein expression and analytical methods.
The research supported by the Molecular Neuroanatomy Core relates to a growing body of evidence that developmental alterations of processes that impact serotonin function during development may lead to a lifetime of chronic mental health problems, including anxiety disorders, learning and emotional disabilities and depression. The Core's activities are vital in understanding the spatial alterations in gene and protein expression as well as morphological changes that arise with perturbations on developmental 5-HT signaling.
|Stewart, Adele; Davis, Gwynne L; Gresch, Paul J et al. (2018) Serotonin transporter inhibition and 5-HT2C receptor activation drive loss of cocaine-induced locomotor activation in DAT Val559 mice. Neuropsychopharmacology :|
|Mayer, Felix P; Schmid, Diethart; Owens, W Anthony et al. (2018) An unsuspected role for organic cation transporter 3 in the actions of amphetamine. Neuropsychopharmacology 43:2408-2417|
|Robson, Matthew J; Quinlan, Meagan A; Margolis, Kara Gross et al. (2018) p38? MAPK signaling drives pharmacologically reversible brain and gastrointestinal phenotypes in the SERT Ala56 mouse. Proc Natl Acad Sci U S A 115:E10245-E10254|
|Brindley, Rebecca L; Bauer, Mary Beth; Walker, L Anne et al. (2018) Adrenal serotonin derives from accumulation by the antidepressant-sensitive serotonin transporter. Pharmacol Res :|
|Knoll, A T; Jiang, K; Levitt, P (2018) Quantitative trait locus mapping and analysis of heritable variation in affiliative social behavior and co-occurring traits. Genes Brain Behav 17:e12431|
|Deneris, Evan; Gaspar, Patricia (2018) Serotonin neuron development: shaping molecular and structural identities. Wiley Interdiscip Rev Dev Biol 7:|
|Brindley, Rebecca L; Bauer, Mary Beth; Blakely, Randy D et al. (2017) Serotonin and Serotonin Transporters in the Adrenal Medulla: A Potential Hub for Modulation of the Sympathetic Stress Response. ACS Chem Neurosci 8:943-954|
|Ritter, K Elaine; Wang, Zunyi; Vezina, Chad M et al. (2017) Serotonin Receptor 5-HT3A Affects Development of Bladder Innervation and Urinary Bladder Function. Front Neurosci 11:690|
|Kast, Ryan J; Wu, Hsiao-Huei; Williams, Piper et al. (2017) Specific Connectivity and Unique Molecular Identity of MET Receptor Tyrosine Kinase Expressing Serotonergic Neurons in the Caudal Dorsal Raphe Nuclei. ACS Chem Neurosci 8:1053-1064|
|O'Neil, Richard T; Wang, Xiaojing; Morabito, Michael V et al. (2017) Comparative analysis of A-to-I editing in human and non-human primate brains reveals conserved patterns and context-dependent regulation of RNA editing. Mol Brain 10:11|
Showing the most recent 10 out of 48 publications