The Conte Bioinformatics and Biostatistics Core has a strong history of close interaction with Conte investigators including the current Center grant funding period, as evidenced by joint publications and the joint development of novel computational algorithms and user-friendly software applications. The Core will continue to sustain the increasing bioinformatics and biostatistics needs of the supported projects and pilot activities. As proposed in the projects, global characterization of epigenomic, transcriptomic, and proteomic changes in response to 5-HT signaling across the life-span will eventually help elucidate the complex molecular mechanisms underlying multiple brain disorders. However, we face tremendous challenges in processing, analyzing, integrating, and interpreting large and heterogeneous datasets generated by multi-level, data rich approaches. A major goal of the Core is to provide computational support to expedite scientific discoveries across multidimensional data sets. We achieve the goal by providing support for data storage, data sharing, and data mining. We utilize a formal data categorization scheme with four data levels that facilitate the location and exchange of data of interest. We provide support for experimental design and statistical data analysis, ensuring that experiments are well planned and powered and pursued with the standards of good statistical design. We perform data analysis using robust, modern statistical models as well as with exploratory graphical techniques. The Core provides support for pathway and network-based data integration and analysis that will be critical for the proposed genome- and transcriptome-wide characterizations of changes induced by developmental 5-HT signaling. The Core plays a critical role in facilitating the integration of data sets generated by individual projects, which can be best achieved through pathway and network-based data analysis and takes advantage of pathway-level statistical analysis and visualization.

Public Health Relevance

The Bioinformatics and Biostatistics Core of the Vanderbilt Conte Center provides expert oversight and creation of approaches to facilitate the integrative and quantitative analysis of large, multidimensional data sets within a framework where tools and analyses can be made readily accessible by the research community at large. The Core is the central node in the Conte Center's network of senior and junior investigators, major and pilot project leaders, where data streams are routed productively to insure effective awareness of modern analytical practices and appropriate statistical methodologies.

Agency
National Institute of Health (NIH)
Institute
National Institute of Mental Health (NIMH)
Type
Specialized Center (P50)
Project #
5P50MH096972-03
Application #
8719819
Study Section
Special Emphasis Panel (ZMH1)
Project Start
Project End
Budget Start
2014-07-01
Budget End
2015-06-30
Support Year
3
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Vanderbilt University Medical Center
Department
Type
DUNS #
City
Nashville
State
TN
Country
United States
Zip Code
37212
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Robson, Matthew J; Zhu, Chong-Bin; Quinlan, Meagan A et al. (2016) Generation and Characterization of Mice Expressing a Conditional Allele of the Interleukin-1 Receptor Type 1. PLoS One 11:e0150068
Margolis, Kara Gross; Li, Zhishan; Stevanovic, Korey et al. (2016) Serotonin transporter variant drives preventable gastrointestinal abnormalities in development and function. J Clin Invest 126:2221-35
Brindley, Rebecca L; Bauer, Mary Beth; Blakely, Randy D et al. (2016) An interplay between the serotonin transporter (SERT) and 5-HT receptors controls stimulus-secretion coupling in sympathoadrenal chromaffin cells. Neuropharmacology 110:438-48
Wu, Hsiao-Huei; Choi, Sera; Levitt, Pat (2016) Differential patterning of genes involved in serotonin metabolism and transport in extra-embryonic tissues of the mouse. Placenta 42:74-83
Goeden, Nick; Velasquez, Juan; Arnold, Kathryn A et al. (2016) Maternal Inflammation Disrupts Fetal Neurodevelopment via Increased Placental Output of Serotonin to the Fetal Brain. J Neurosci 36:6041-9
Ellegood, J; Anagnostou, E; Babineau, B A et al. (2015) Clustering autism: using neuroanatomical differences in 26 mouse models to gain insight into the heterogeneity. Mol Psychiatry 20:118-25

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