The Neurochemistry Core is a dual function core that will support the research efforts ofthe Projects by providing services while at the same time developing new tools and datasets that will ultimately inform each ofthe Projects. The core will provide basic histology services to document probe placements for Projects 1 and 2, and will perform quantitative oxytocin receptor (OXTR) autoradiography in rats and voles for Project 1. Th13 Core will also provide Projects 2 and 3 with novel potent and selective OXTR antagonists for their studies in nonhuman primates. In addition, the Core will play a critical role in delineating the neuroanatomical localization of OXTR in the primate brain (currently not understood). This knowledge is essential for understanding the neural mechanisms by which oxytocin (OT) modulates social cognition in Projects 2 and 3. We will use a three pronged approach for mapping the OXTR in rhesus monkey brain. First, a validated RT-PCR technique will be used to map the location of OXTR in amygdala (Amy) subnuclei as well as to determine the neuronal phenotype of OXTR neurons after single cell recording. Second, we will work to improve the selectivity of receptor autoradiography procedures in primate tissue. Finally we will continue our effort to develop and validate novel PET ligands for in vivo imaging of OXTR in living brains. If successful, the PET ligands will contribute directly to the goals of Project 2 and 3 and may ultimately be used to inform the human studies in Project 4.
These aims and the functions and services that they support are essential for the integration of research designs and interpretation of findings across projects and will provide for the first time an understanding of the neural distribution of OXTR in the primate brain. The novel OXTR antagonists and PET'ligands will have a tremendous benefit for the entire research community investigating the role of OT on social behavior.
The core will provide functions and services to each Project (including the development of novel compounds and technologies), essential for the integration of designs and findings across projects, and will provide for the first time with an understanding of the localization of OXTR in primate brain. The novel OXTR antagonists and PET ligands will have a strong impact in the field to study OT role on social primate behavior.
|Modi, Meera E; Connor-Stroud, Fawn; Landgraf, Rainer et al. (2014) Aerosolized oxytocin increases cerebrospinal fluid oxytocin in rhesus macaques. Psychoneuroendocrinology 45:49-57|
|Freeman, Sara M; Inoue, Kiyoshi; Smith, Aaron L et al. (2014) The neuroanatomical distribution of oxytocin receptor binding and mRNA in the male rhesus macaque (Macaca mulatta). Psychoneuroendocrinology 45:128-41|
|Mosher, Clayton P; Zimmerman, Prisca E; Gothard, Katalin M (2014) Neurons in the monkey amygdala detect eye contact during naturalistic social interactions. Curr Biol 24:2459-64|
|Freeman, S M; Walum, H; Inoue, K et al. (2014) Neuroanatomical distribution of oxytocin and vasopressin 1a receptors in the socially monogamous coppery titi monkey (Callicebus cupreus). Neuroscience 273:12-23|
|Rilling, James K; Young, Larry J (2014) The biology of mammalian parenting and its effect on offspring social development. Science 345:771-6|
|Skuse, David H; Lori, Adriana; Cubells, Joseph F et al. (2014) Common polymorphism in the oxytocin receptor gene (OXTR) is associated with human social recognition skills. Proc Natl Acad Sci U S A 111:1987-92|
|König, Seth D; Buffalo, Elizabeth A (2014) A nonparametric method for detecting fixations and saccades using cluster analysis: removing the need for arbitrary thresholds. J Neurosci Methods 227:121-31|