Postmortem human brain tissue is essential for studies of the pathobiology and causes of Parkinson's disease (PD). The chief goal of the Clinical &Neuropathology Core (Core D) is to obtain and provide well-characterized postmortem human brain tissue for investigations of the etiology and basic mechanisms of Parkinson's disease (PD). These tissues are necessary to validate the hypotheses and observations generated by the studies of cellular and molecular biology and experimental animal models proposed in Projects 1-3. Core D clinical assessment protocols in concert with its neuropathological analyses and laboratory facilities enable acquisition of clinical data, brain tissue, and genetic material from the same subject and serve as powerful resources for validating findings from the Center's cellular and transgenic model studies. Accordingly, the staff of Core D recruits and follows clinically patients with PD and related disorders, as well as controls. These subjects give consent for eventual autopsy and brain donation and are enrolled in a prospective autopsy cohort. At death, the Core arranges and performs autopsies and provides neuropathological diagnoses in these subjects. Postmortem tissues are prepared and archived both for diagnostic and research purposes by the Brain Resource Center (BRC). The BRC serves as a repository of fixed and frozen brain tissues prepared for research including cases of PD, other Lewy body diseases, and normal and diseased controls. Core D also maintains and staffs histology, immunocytochemistry and stereology laboratories to support and facilitate the morphological assessment of human and experimental mouse models relevant to the studies of PD delineated in Projects 1-3. Finally, Core D provides the fundamental infrastructure to facilitate independently funded clinical and clinicopathological investigations of PD at JHMI and collaborating institutions and trains basic investigators and clinical neuroscientists in clinical and neuropathology issues relevant to PD.

Public Health Relevance

The Clinical and Neuropathology Core serves as a resource for all of the research projects in the PD Center. Its main goal is to conduct clinical assessments and postmortem examination of patients with PD. The Core provides postmortem brain tissues to all of the Projects in the Hopkins PD Center and to investigators in other Udall Centers. The availability of these tissue samples is a critical step in the investigation of the causes and underlying mechanisms of PD.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Specialized Center (P50)
Project #
5P50NS038377-14
Application #
8380734
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
2012-08-01
Budget End
2013-07-31
Support Year
14
Fiscal Year
2012
Total Cost
$530,221
Indirect Cost
$206,914
Name
Johns Hopkins University
Department
Type
DUNS #
001910777
City
Baltimore
State
MD
Country
United States
Zip Code
21218
Hinkle, Jared T; Perepezko, Kate; Rosenthal, Liana S et al. (2017) Markers of impaired motor and cognitive volition in Parkinson's disease: Correlates of dopamine dysregulation syndrome, impulse control disorder, and dyskinesias. Parkinsonism Relat Disord :
Panicker, Nikhil; Dawson, Valina L; Dawson, Ted M (2017) Activation mechanisms of the E3 ubiquitin ligase parkin. Biochem J 474:3075-3086
Dawson, Ted M; Dawson, Valina L (2017) Mitochondrial Mechanisms of Neuronal Cell Death: Potential Therapeutics. Annu Rev Pharmacol Toxicol 57:437-454
Yun, Seung Pil; Kim, Hyojung; Ham, Sangwoo et al. (2017) VPS35 regulates parkin substrate AIMP2 toxicity by facilitating lysosomal clearance of AIMP2. Cell Death Dis 8:e2741
Sulzer, David; Alcalay, Roy N; Garretti, Francesca et al. (2017) T cells from patients with Parkinson's disease recognize ?-synuclein peptides. Nature 546:656-661
Liddelow, Shane A; Guttenplan, Kevin A; Clarke, Laura E et al. (2017) Neurotoxic reactive astrocytes are induced by activated microglia. Nature 541:481-487
Xiong, Yulan; Dawson, Ted M; Dawson, Valina L (2017) Models of LRRK2-Associated Parkinson's Disease. Adv Neurobiol 14:163-191
Gwinn, Katrina; David, Karen K; Swanson-Fischer, Christine et al. (2017) Parkinson's disease biomarkers: perspective from the NINDS Parkinson's Disease Biomarkers Program. Biomark Med 11:451-473
Ando, Maya; Fiesel, Fabienne C; Hudec, Roman et al. (2017) The PINK1 p.I368N mutation affects protein stability and ubiquitin kinase activity. Mol Neurodegener 12:32
Lee, Yunjong; Stevens, Daniel A; Kang, Sung-Ung et al. (2017) PINK1 Primes Parkin-Mediated Ubiquitination of PARIS in Dopaminergic Neuronal Survival. Cell Rep 18:918-932

Showing the most recent 10 out of 228 publications